- Title
-
Intestinal Upregulation of Melanin-Concentrating Hormone in TNBS-Induced Enterocolitis in Adult Zebrafish
- Authors
- Geiger, B.M., Gras-Miralles, B., Ziogas, D.C., Karagiannis, A.K., Zhen, A., Fraenkel, P., and Kokkotou, E.
- Source
- Full text @ PLoS One
Histological features of TNBS-induced enterocolitis in adult zebrafish. A) Representative H&E stained sections form TNBS- or vehicle (30% ethanol) treated animals, at two different magnifications. Arrowheads indicate villi thickness in TNBS- and vehicle-treated intestinal sections. A region showing luminal sloughing of cellular debris in the TNBS-treated intestine is marked by a dotted line. B-C). Relative villi thickness and height (in mm) were evaluated as described in Methods. D) Abundance of goblet cells based on a 0-5 scoring system. PHENOTYPE:
|
MCH intestinal upregulation in TNBS-induced enterocolitis in adult zebrafish. A) Relative mRNA expression of the two different MCH peptides MCH1 and MCH2 in the zebrafish intestine following 6 hours of TNBS intrarectal infusion. TBP serves as the housekeeping gene. (n=9-10 fish per group). For each gene, expression in the vehicle treated group has been set to 100. AU: arbitrary mRNA units. B) Immunofluorescence analysis of MCH positive cells in the zebrafish intestine at baseline and in response to TNBS treatment. An antibody against human MCH was used for staining zebrafish MCH. |