Campbell et al., 2013 - Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development. Gene expression patterns : GEP   13(7):271-279 Full text @ Gene Expr. Patterns

Fig. 3 (A) Temporal analysis of zebrafish kif5s during development by RT-PCR. (B) RT-PCR analysis of zebrafish kif5 tissue and organ specific expression. Asterisk () indicates faint bands present. NRT, no reverse transcriptase.

Fig. 4 kif5 expression during early zebrafish development. Maternal expression of kif5s was apparent in cleavage stage for kif5Ba, kif5Bb, and kif5C, but not kif5Aa (A–D). Expression of kif5s at sphere (E–H), shield (I–L), bud (M-P), 15 somite (Q–T), and 19 somite (U–X) stages. Arrowheads indicate trigeminal ganglion and arrows indicate otic placode. All images are lateral views. For A–L, An = Animal pole, Vg = vegetal pole. For M–X, A = Anterior, P = Posterior, V = Ventral, D = Dorsal. Dashed line represents border between notochord and spinal cord. fb, forebrain; mb, midbrain; hb, hindbrain; ey, eye.

Fig. 5 Expression of kif5s at 24 hpf and 36 hpf. (A–E) Lateral views of whole mount in situs at 24 hpf. kif5Aa, kif5Ab, and kif5C were detected in the telencephalon (te), diencephalon (di), and hindbrain (hb). kif5Aa and kif5C were also detected in the tegmentum (tg), epiphysis (ep), and spinal cord (sc). kif5Ba and kif5Bb were ubiquitously expressed. (F, G) Dorsal view of flat mounts at 24 hpf. kif5Aa and kif5C were detected in the midbrain-hindbrain boundary (MHB) and trigeminal ganglia (TGg). kif5Aa was also detected in the posterior lateral line ganglia (PLLg) while kif5C was more broadly expressed in other tissues. (H, I) Dorsal view of flat mounts at 36 hpf. Anterior is to the left, dorsal to the top for A–E. Anterior is to the left for F–I. MHB, midbrain–hindbrain boundary.

Fig. 6 Expression of kif5s at 48 hpf and 96 hpf. (A,C,E,G and, I) Lateral views of whole mount in situs at 48 hpf. kif5Aa, kif5Ab, and kif5C were detected throughout the central nervous system while kif5Bb and kif5Bb were ubiquitously expressed. (B,D,F,H and, J) Lateral views of whole mount in situs at 96 hpf. kif5Aa, kif5Ab, and kif5C continued to be expressed in the central nervous system. kif5Ba and kif5Bb were ubiquitously expressed and showed robust expression in the digestive tract (dt). A = Anterior, P = Posterior, V = Ventral, D = Dorsal. fb, forebrain; mb, midbrain; hb, hindbrain; n, nose; ey, eye.

Fig. 7 (A–E) Expression of kif5s in the retina at 96 hpf. White brackets show the expression domain of each individual kif5. (F) Diagram of zebrafish retinal organization. (G) Table summarizes the relative abundance of each individual kif5 across the retinal layers.

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Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 13(7), Campbell, P.D., and Marlow, F.L., Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development, 271-279, Copyright (2013) with permission from Elsevier. Full text @ Gene Expr. Patterns