Lin et al., 2012 - Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio). PLoS One   7(9):e45650 Full text @ PLoS One

Fig. 2

Effects of exogenous 1α,25(OH)2D3 (20 µg/l) in 3-d post-fertilization (dpf) zebrafish embryos.

Ca2+ influx (A), Ca2+ content (B), mRNA expressions in 3-dpf zebrafish embryos (C). mRNA expressions were analyzed by a qPCR, and values were normalized to β-actin. Values are the mean ± SD (n = 7~10). Student’s t-test, *p<0.05; **p<0.01, ***p<0.001.

EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: 1-cell to Protruding-mouth

Fig. 4 Zebrafish vdra and vdrb expression profiles.

mRNA expression patterns in developing stages (A) and various tissues (B) were determined by an RT-PCR using β-actin as the internal control. (C) vdra and vdrb mRNA expressions in different tissues of adult zebrafish were analyzed by a qPCR using β-actin as the internal control. Values are the mean ± SD (n = 3). E, eye; K, kidney; Sk, skin; I, intestine; G, gill; H, heart; M, muscle; B, brain; L, liver; Ov, ovary; Te, testis.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult

Fig. 5 Effects of vitamin D receptor (VDR)a and VDRb morpholino oligonucleotides (MOs) in 3-d post-fertilization (dpf) zebrafish embryos.

Ca2+ influx (A), Ca2+ content (B), mRNA expression (C), density of ecac-expressing cells (D), and ecac signals (E). mRNA expressions were analyzed by a qPCR using β-actin as the internal control. Different letters indicate a significant difference (p<0.05) using a one-way ANOVA followed by Tukey’s multiple-comparison test. Values are the mean ± SD (n = 6 or 7). Scale bar:100 μm.

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 7

Effects of vitamin D receptor (VDR)a and VDRb morpholino oligonucleotides (MOs) on cyp27b1 and cyp24a1 mRNA expressions in 3-d post-fertilization (dpf) zebrafish embryos treated with 1α,25(OH)2D3 (20 µg/l).

(A) cyp27b1 mRNA expression. (B) cyp24a1 mRNA expression. mRNA expressions were analyzed by a qPCR using β-actin as the internal control. Different letters indicate a significant difference (p<0.05) using one-way ANOVA followed by Tukey’s multiple-comparison test. Values are the mean ± SD. (n = 5-6).

EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth

Fig. S1 Specificity and effectiveness of vitamin D receptor (VDR)a and VDRb morpholino oligonucleotides (MOs). VDRa and VDRb MOs were respectively injected into 1- or 2-cell embryos. To clarify the MO specificity and effectiveness, Western blotting was used to detect VDRa and VDRb protein expressions in wild-type (WT) and MO-injected embryos at 3 d post-fertilization (dpf). An asterisk (*) indicates the position of VDRa or VDRb expression.

Fig. S2 Effect of vitamin D receptor (VDR)a and VDRb morpholino oligonucleotides (MOs) on ossification of vertebrae of 5-d post-fertilization (dpf) zebrafish embryos. Control (A), VDRa (B), and VDRb (C) MO. Ossification of vertebrae was observed by calcein staining. Scale bar: 100 μm.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. S3 Colocalization of vdra mRNA with Na,K-ATPase rich (NaR) cells in zebrafish gill cryosections. (A) In situ hybridization of vdra mRNA; (B) immunocytochemical staining of Na,K-ATPase. The arrow indicates colocalization of vdra mRNA and Na,K-ATPase protein signals in the same cells. Scale bar: 5 μm.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Adult
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One