Fig. 4

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EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult
Fig. 8

Subcellular localization of zebrafish R2 isoforms.

(A) Immunofluorescence staining was performed to detect the subcellular distribution of zebrafish R2 isoforms in transfected Hela cells. Three R2 isoforms were tagged with a Flag at N-terminus. At 36 h after transfection, R2 isoforms were detected with primary anti-Flag antibody and FITC-conjugated secondary antibody. Nuclei were stained with DAPI. (B) Co-localization of fluorescent protein-tagged zebrafish R1 and R2 isoforms in the cytosol of Hela cells. R2 isoforms or R1 of zebrafish were fused with GFP or RFP to their C-termini. At 36 h after transfection, images were directly acquired under fluorescence microscopy.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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