Moon et al., 2011 - Fucoidan promotes mechanosensory hair cell regeneration following amino glycoside-induced cell death. Hearing Research   282(1-2):236-42 Full text @ Hear. Res.

Fig. 1 Expression of fluorescence in transgenic zebrafish. Transgenic pou4f3::GFP larvae displayed GFP on hair cells; scm1::GFP larvae, on the internal neuromast supporting cells; and ET20::GFP larvae, on peripheral neuromast supporting cells.

Fig. 2 The effect of LMWF on hair cell regeneration. (A) Hair cells in the lateral line labeled with YO-PRO-1were eliminated in zebrafish 4 h after neomycin (500 μM) exposure. The number of trunk neuromasts and hair cells per neuromast were markedly increased in the LMWF treatment group 12 h after neomycin exposure. Insets are close views of P8 neuromasts. (B) Summary data from 10 independent experiments (p < 0.001) compared to neomycin only. (C) A regenerated hair cell stained with FM1-43. (NM; Neomycin, LMWF; low molecular weight foucoidan, * p < 0.05, scale bar; 10 μM).

Fig. 3 Scanning electron microscopy of hair cells in neuromasts. Stereocilia bundles on hair cells were still destroyed 24 h after neomycin (500 μM) treatment, but regenerated in the presence of LMWF 24 h after neomycin treatment (×5000).

Fig. 4 The effect of LMWF on hair regeneration after neomycin-induced ototoxicity in POU4f3::GFP larvae. (A) A greater number of hair cells was observed in the neuromasts (P8) of LWMF-treated larvae 12 h after neomycin treatment. (B) Summarized data from six independent experiments. LMWF (50 μg/ml) significantly enhanced hair cell regeneration 12 and 16 h after neomycin treatment. (C) Concentrations of LMWF above 50 μg/ml had similar effects on hair cell regeneration (p < 0.001 compared to neomycin only).

Fig. 5 The effect LMWF on the proliferation of supporting cells. Greater numbers of internal supporting cells in scm1::GFP larvae and peripheral supporting cells in ET20::GFP larvae were observed after LMWF treatment (quantification was not done). Each figure shows P7 neuromasts 12 h after neomycin (500 μM) treatment.

Fig. 6 BrdU incorporation after neomycin treatment in POU4f3::GFP larvae. (A) More cells are positive for BrdU (red color) in the LMWF group 12 h after neomycin treatment. (B) Summary of data from six experiments. Neuromast cell entry into the S-phase increased 8, 12, 16, and 24 h after neomycin treatment in LMWF treat group (p < 0.001, n = 10; compared to neomycin only). (C) BrdU-labeled cells (red dots) are generally located at the periphery of hair cells (green color) in POU4f3::GFP larvae and are centered over the peripheral supporting cells in ET20::GFP larvae. (NM; Neomycin, LMWF; low molecular weight foucoidan, * p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Acknowledgments:
ZFIN wishes to thank the journal Hearing Research for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Hearing Research, 282(1-2), Moon, I.S., So, J.H., Jung, Y.M., Lee, W.S., Kim, E.Y., Kim, C.H., and Choi, J.Y., Fucoidan promotes mechanosensory hair cell regeneration following amino glycoside-induced cell death, 236-42, Copyright (2011) with permission from Elsevier. Full text @ Hear. Res.