Chauvigné et al., 2011 - Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol. BMC Biotechnology   11:34 Full text @ BMC Biotechnol.

Fig. 4 Functional characterization of DrAqp3b mutants. (A) Pf of control oocytes (water-injected) and oocytes expressing 1 ng cRNA encoding wild-type DrAqp3b (DrAqp3b-WT) or different DrAqp3b mutants at different pH. Values are the mean ± SEM of three experiments (n = 8-10 oocytes per construct). The asterisks denote significant differences between WT and mutant DrAqp3b at a given pH (Student′s t test, p < 0.05). (B) Immunofluorescence microscopy of water-injected oocytes (control), or oocytes expressing DrAqp3b-WT, -H53A, -H53A/G54H, -T85A and -H53A/G54H/T85A using an affinity purified anti-DrAqp3b antiserum. The arrowhead points to the oocyte plasma membrane. (C) Immunoblot of total membrane protein extracts of control oocytes or oocytes expressing DrAqp3b-WT treated or not with N-glycosidase F (PNGase F). The arrows indicate glycosylated and deglycosylated forms of DrAqp3b-WT. (D) Representative immunoblot of plasma membrane protein extracts of oocytes expressing increasing amounts of cRNA (1-8 ng) encoding DrAqp3b-WT, -T85A and -H53A/G54H/T85A treated with PNGaseF.

ZFIN wishes to thank the journal BMC Biotechnology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biotechnol.