Tseng et al., 2011 - Exploring Uncoupling Proteins and Antioxidant Mechanisms under Acute Cold Exposure in Brains of Fish. PLoS One   6(3):e18180 Full text @ PLoS One

Fig. 6 zucp mRNA expressions in various tissues of zebrafish.

Isoform-specific primer sets were used for RT-PCR analysis. rpl13a was used as the internal control.

Fig. 7 mRNA in situ hybridization of zucp paralogs in brains′ cross section of adult zebrafish.

All the five zucps were observed in diencephalon, mescencephalon, and cerebellum. In addition, except the zucp5, zucp1, -2, -2l, -4 were all expressed in medulla oblongata. Besides, zucp2 mRNA also distributed in telencephalon. Detailed mRNA expression patterns of zucps in zebrafish brain were listed in Table 2. CC, cerebellar crest; CCe, cerebellar corpus; CP, central posterior thalamic nucleus; DP, dorsal posterior thalamic nucleus; EG, granular eminence; Ha, habenula; LCa, caudal lobe of cerebellum; PGZ, periventricular gray zone of optic tectum; PPa, parvocellular preoptic nucleus; TL, longitudinal torus; TPp, periventricular nucleus of posterior tuberculum; Val, lateral division of valvula cerebelli; Vam, valvula crebelli; Vl, lateral nucleus of ventral telecephalic area; VM, ventromedial thalamic nucleus.

Fig. 8

UCP expressions in zebrafish brain.

qPCR analysis of 5 zucp isoforms in brains of zebrafish at 28°C and 1, 6 or 24 h after transfer to 18°C. rpl13a was used as the internal control. Data are presented as mean ± SD (N = 4). Different letters indicate significant differences between treatments (one-way ANOVA, Tukey′s pairwise comparison, p<0.05).

EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult

Fig. 11

Catalase expressions in zebrafish brain.

qPCR analysis of catalase (zcat) mRNA expressions in brains of zebrafish at 28°C and 1, 6 or 24 h after transfer to 18°C. rpl13a was used as the internal control. Data are presented as mean ± SD (N = 4). Different letters indicate significant differences between treatments (one-way ANOVA, Tukey′s pairwise comparison, p<0.05).

EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Adult

Fig. 13

PPAR expressions in zebrafish brain.

qPCR analysis of different types and isoforms of zppar mRNA expressions in zebrafish brains at 28°C and 1, 6 or 24 h after transfer to 18°C. (A) zpparαa mRNA, (B) zpparαb mRNA, (C) zpparδa mRNA, (D) zpparδb mRNA, (E) zpparγ mRNA. rpl13a was used as the internal control. Data are presented as mean ± SD (N = 4). Different letters indicate significant differences between treatments (one-way ANOVA, Tukey′s pairwise comparison, p<0.05).

EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult

Fig. 15

Expressions of GLUT in zebrafish brain.

Comparison of zglut1a (A) and zglut3 (B) transcripts (by qPCR) in adult zebrafish brains at 28°C and 1 or 24 h after transfer to 18°C. Data are presented as the mean ± SD (N = 4). Different letters indicate significant differences between treatments (one-way ANOVA, Tukey′s pairwise comparison, p<0.05).

EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One