Gallardo et al., 2010 - Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish. BMC Developmental Biology   10:120 Full text @ BMC Dev. Biol.

Fig. 1 Section of the transgenic line cldnb:gfp. (A) Schematic drawings of a migrating primordium of the posterior lateral line along the horizontal myoseptum at 36 hpf. (B) Transgenic cldnb:gfp embryo at 36 hpf, when tails were sectioned (red line). (C) Sectioned tails with GFP primordium. (d) RT-PCR of RNA derived from dissected tails of 36 hpf embryos with primer specific to three genes known to be expressed in the primordium at this stage. L1: neuromast 1, L2: neuromast 2, prim: LLP primordium. Scale bars are 50 μm in (b-c).

Fig. 4 Validation of microarray analysis. (A) Quantitative RT-PCR for individual genes with different biological roles was performed of RNA derived from GFP+ and GFP- cells from tails of 36 hpf embryos. Real time PCR ratios were determined by normalization to β-actin (equal to 1, dotted line). Only 3 genes out of the 15 tested were not significantly enriched (asterisks). (B-G) In situ hybridization of 6 genes enriched in GFP+ cells showing a primordium specific expression pattern in 36 hpf embryos. (H-O) Loss-of-function analysis on two selected genes (cd9b and f11r) enriched in GFP+ cells of 36 hpf embryos. Cldnb:gfp embryos were injected with anti-sense morpholinos (MO) against cd9b (I, L-M) and f11r (J, N-O), and compared to control (H, K). White arrows indicate the position where the primordium is at 36 hpf (H-J). Red arrowheads indicate the rosette-like structures in the primordium (K). Scale bars are 10 μm in (B-G) and (K-O).

Fig. S4 Pattern of the neuromast deposition in the embryonic PLL. The number and position of the neuromasts was analyzed in control (A), cd9bMO (B-C), and f11rMO (D-E) embryos at 48 hpf. Red arrows indicate the position of the PLL neuromasts.

ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.