- Title
-
Positional cloning of "Lisch-Like", a candidate modifier of susceptibility to type 2 diabetes in mice
- Authors
- Dokmanovic-Chouinard, M., Chung, W.K., Chevre, J.C., Watson, E., Yonan, J., Wiegand, B., Bromberg, Y., Wakae, N., Wright, C.V., Overton, J., Ghosh, S., Sathe, G.M., Ammala, C.E., Brown, K.K., Ito, R., LeDuc, C., Solomon, K., Fischer, S.G., and Leibel, R.L.
- Source
- Full text @ PLoS Genet.
Expression Patterns and Morpholino Knockdown in Zebra Fish Embryos. A) Developmental expression of zebra fish Lisch-like and Lsr-like orthologs. Lisch-like RNA was hybridized in situ to whole-mount zebra fish embryos at 48 hours post-fertilization (hpf), dorsal view with anterior towards the top; and 72 hpf, lateral view with anterior towards the top, ventral towards the right and yolk removed. Lsr-like RNA was hybridized at 48 hpf and 34 hpf. Ll panels show ventral views of embryos with yolks removed and anterior towards the top. Lsr-like panels show the same image captured in the focal plane of the anterior (ap) and posterior (pp) pancreatic buds, respectively. i, intestine; ph, pharynx; pn, pronephric ducts; l, liver; ap, anterior pancreatic bud; pp, posterior pancreatic bud; p, pancreas (after anterior and posterior bud fusion); b, brain; o, otic vesicle. B) Morpholino knockdown of Lisch-like and Lsr-like orthologs at 48 hpf. Two dimensional ventral views (anterior towards top) of confocal stacks of 48 hpf embryos, uninjected or injected with 15 ng morpholino: control, Lsr-like sp1, and Lisch-like ATG. Gut-GFP transgene expression (green); insulin immunolabelling (red). EXPRESSION / LABELING:
PHENOTYPE:
|
Unillustrated author statements EXPRESSION / LABELING:
PHENOTYPE:
|