FIGURE SUMMARY
Title

E-box function in a period gene repressed by light

Authors
Vallone, D., Gondi, S.B., Whitmore, D., and Foulkes, N.S.
Source
Full text @ Proc. Natl. Acad. Sci. USA

RNase protection assay analysis of zfper4 (410-nt protected fragment, amino acid positions 88–225) and β-actin (282-nt protected fragment) was performed with RNA harvested at the indicated zeitgeber times (ZT0 is lights-on, ZT12 is lights-off) or CT. (A) Whole brain from adult zebrafish maintained in LD. (B) Six-day-old larvae raised in LD. (C) PAC-2 cells were maintained in LD for 5 days. Cells were entrained in LD and harvested in DD (D)orinLL(E). (F) Peak and trough samples from the LD, DD, and LL sets of PAC-2 cells (C–E) assayed together to allow comparison of the relative expression levels. (G) Larvae raised for 6 days in DD and then exposed to light for the indicated times (hours; + Light). Control larvae remained in DD and were harvested in parallel. (H) Analysis of PAC-2 cells equivalent to G.

EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Day 6 to Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA