Thisse et al., 2001 - Expression of the zebrafish genome during embryogenesis (NIH R01 RR15402). ZFIN Direct Data Submission
Submitted By: Thisse, Bernard, Thisse, Christine(Citing this work)
Thisse in situ hybridization protocol
Probe: cb260   Quality: Rating 5
Supplier: Zebrafish International Resource Center (ZIRC) (order this)

Fig. 1 First expression is observed at 70% epiboly in dorsal neural plate, in territory lining the presumptive epidermis from the animal pole to the ventral territory near the margin (a territory which corresponds to the presumptive dorsal spinal cord). Ventral proneural clusters appear later (80% epiboly) first near the margin in a territory corresponding to the ventral spinal cord. At the same developmental stage, strong expression is observed at the level of the presumptive midbrain.

EXPRESSION / LABELING:
Gene: neurog1
Fish: AB/TU
Anatomical Terms: ectoderm, neural keel, neural plate, presumptive brain
Stage Range: 50%-epiboly to Bud

Fig. 2 Expression specific of proneural clusters at the border of the neural plate as well as in ventral neural plate territory.

EXPRESSION / LABELING:
Gene: neurog1
Fish: AB/TU
Anatomical Terms: cranial ganglion, ectoderm, forebrain neural keel, hindbrain neural plate, midbrain neural keel (all 11) expand
Stage Range: 1-4 somites to 10-13 somites

Fig. 3

EXPRESSION / LABELING:
Gene: neurog1
Fish: AB/TU
Anatomical Terms: central nervous system, cranial ganglion, diencephalon, ectoderm, forebrain (all 13) expand
Stage: 14-19 somites

Fig. 4

EXPRESSION / LABELING:
Gene: neurog1
Fish: AB/TU
Anatomical Terms: alar plate midbrain region, basal plate midbrain region, central nervous system, cranial ganglion, diencephalon (all 18) expand
Stage Range: 20-25 somites to Prim-5

Fig. 5

EXPRESSION / LABELING:
Gene: neurog1
Fish: AB/TU
Anatomical Terms: central nervous system, cranial ganglion, diencephalon, forebrain, hindbrain (all 15) expand
Stage Range: Prim-15 to Prim-25

Fig. 6 in addition to the expression in neural cell (expression linked to neurogenesis) ngn1 transcripts are also observed in proximal part of the endodermal pouches

EXPRESSION / LABELING:
Gene: neurog1
Fish: AB/TU
Anatomical Terms: central nervous system, diencephalon, forebrain, hindbrain, midbrain (all 13) expand
Stage Range: High-pec to Long-pec