Fig. 1 Expression of Antivin1 starts at blastula stage, immediately after initiation of zygotic genome. Expression in cells of the dorsal margin. Expression extends rapidly to encompass the whole blastula margin at late blastula stage.

Fig. 2 At the beginning of gastrulation expression decreases transiently at the dorsal margin then strongly increases in involuted axial mesoderm. The marginal expression decreases progressively and is no longer observed at late gastrula stage. In the axis expression initially restricted to the axial mesoderm starts to be observed in ventral neuroectoderm, first in cells immediately adjacent to the migrating axial mesoderm. At the end of gastrulation, expression decreases strongly in antero-axial mesoderm and is only observed in presumptive ventral forebrain.

Fig. 3 Expression in presumptive ventral forebrain. By the 7 somite stage, a new site of expression is observed in posterior cells of the notochord. The forebrain expression disappears and the notochordal staining enlarges from a few cells posteriorly to the complete notochord by the 12 somite stage.

Fig. 4 The notochordal expression starts to disappear from the posterior third of the notochord. The notochordal domain in which antivin stops being expressed extends rapidly both anteriorly and posteriorly. The caudal tip of the notochord maintains expression of antivin until the end of tail elongation. Two asymmetrical domains of expression appear in the left heart primordium as well as in the left part of the dorsal diencephalon. In 4.5% of embryos expression in heart primordium and in dorsal diencephalon is bilaterally symmetrical.

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