Transgenic Construct

Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus)

ID
ZDB-TGCONSTRCT-151028-2
Name
Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus)
Previous Names
  • Tg(hsp70l:cdc25_S213A/S305A/T564A,Venus) (1)
  • Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-FMA)
  • Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
[Em λ][Ex λ]
Fpbase:Venus
Contains Sequences
Note
The hsp70l promoter drives transcription of cdc25 (NM_001115095.2) and Venus as a single transcript followed by an SV40 poly-A sequence. Cdc25 and Venus are separated by a viral 2A peptide sequence (Provost, et al. 2007, Genesis. 45, p. 625-629). The construct is flanked by Tol2 recognition sites. Note that this form of cdc25 has the following mutations: S213A, S305A and T564A
Plasmid Map
None
Genomic Features
That Utilize Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus) Construct
Genomic Feature Affected Genomic Regions
w132Tg
Transgenics
That Utilize Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus) Construct
Fish Affected Genomic Regions Phenotype Gene Expression
w132Tg
w132Tg/+ (AB)
Sequence Information
Other Construct Pages
None
Citations