Transgenic Construct
Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus)
- ID
- ZDB-TGCONSTRCT-151028-2
- Name
- Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus)
- Previous Names
-
- Tg(hsp70l:cdc25_S213A/S305A/T564A,Venus) (1)
- Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-FMA)
- Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus)
- Type
- engineered_plasmid
- Regulatory Regions
- Coding Sequences
- [Em λ][Ex λ]
- Fpbase:Venus
- Contains Sequences
- Note
-
The hsp70l promoter drives transcription of cdc25 (NM_001115095.2) and Venus as a single transcript followed by an SV40 poly-A sequence. Cdc25 and Venus are separated by a viral 2A peptide sequence (Provost, et al. 2007, Genesis. 45, p. 625-629). The construct is flanked by Tol2 recognition sites. Note that this form of cdc25 has the following mutations: S213A, S305A and T564A
Plasmid Map
None
Genomic Features
That Utilize Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus) Construct
Genomic Feature | Affected Genomic Regions |
---|---|
w132Tg |
Transgenics
That Utilize Tg(hsp70l:cdc25b_S213A_S305A_T564A-2A-Venus) Construct
Fish | Affected Genomic Regions | Phenotype | Gene Expression |
---|---|---|---|
w132Tg |
|
||
w132Tg/+ (AB) |
|
Sequence Information
Other Construct Pages
None
Citations