Transgenic Construct

Tg(mitfa:kcnj13)

ID
ZDB-TGCONSTRCT-120306-2
Name
Tg(mitfa:kcnj13)
Previous Names
  • Tg(mitfa:DsRed-kcnj13)
  • Tg(mitfa:kcnj13)kcnj13b230/b230 (1)
Type
engineered_plasmid
Regulatory Regions
Coding Sequences
[Em λ][Ex λ]
Fpbase:DsRed
Contains Sequences
Note
"(mitfa:DsRed-kcnj13) was prepared by modifying pTol2-mitfa:DsRed plasmid, which is named as pTol2-nacre-DsRed in the paper. To make this plasmid; mitfa:DsRed-kcnj13, kcnj13 gene was integrated between mitfa-promoter and DsRed gene of pTol2-mitfa:DsRed plasmid and the kcnj13 included it own stop codon. Single mRNA on which kcnj13 and DsRed genes are harbored are expressed under the mitfa promoter. Because kcnj13 has stop codon and there is not promoter sequence for DsRed, DsRed is not translated to protein." (Masakatsu Watanabe)
Plasmid Map
None
Genomic Features
That Utilize Tg(mitfa:kcnj13) Construct
Genomic Feature Affected Genomic Regions
ou2101Tg
Transgenics
That Utilize Tg(mitfa:kcnj13) Construct
Fish Affected Genomic Regions Phenotype Gene Expression
kcnj13db230/db230; ou2101Tg Fig. S1 from Inaba et al., 2012
Sequence Information
Other Construct Pages
None
Citations