PUBLICATION

Zebrafish YAC, BAC, and PAC genomic libraries

Authors
Amemiya, C.T., Zhong, T.P., Silverman, G.A., Fishman, M.C., and Zon, L.I.
ID
ZDB-PUB-990309-405
Date
1999
Source
In The Zebrafish: Genetics and Genomics, H.W. Detrich, III, M. Westerfield, and L.I. Zon, eds., San Diego, CA: Academic Press, Methods Cell Biol.   60: 235-258 (Review)
Registered Authors
Amemiya, Chris, Fishman, Mark C., Zhong, Tao P., Zon, Leonard I.
Keywords
none
MeSH Terms
  • Animals
  • Bacteria
  • Chromosomes, Artificial, Yeast*
  • Cloning, Molecular
  • Genetic Testing/methods
  • Genomic Library*
  • Humans
  • Internet
  • Mice
  • Zebrafish/genetics*
PubMed
9891341
Abstract
Numerous positional cloning projects directed at isolating genes responsible for the myriads of observed developmental defects in the zebrafish are anticipated in the very near future. In this chapter, we have reviewed the YAC, BAC, and PAC large-insert genomic resources available to the zebrafish community. We have discussed how these resources are screened and used in a positional cloning scheme and have pointed out frequently formidable logistical considerations in the approach. Despite being extremely tedious, positional cloning projects in the zebrafish will be comparatively easier to accomplish than in human and mouse, because of unique biological advantages of the zebrafish system. Moreover, the ease and speed at which genes are identified and cloned should rapidly increase as more mapping reagents and information become available, thereby paving the way for meaningful biological studies.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes