PUBLICATION

Identification of three putative GnRH receptor subtypes in vertebrates

Authors
Troskie, B., Illing, N., Rumbak, E., Sun, Y.M., Hapgood, J., Sealfon, S., Conklin, D., and Millar, R.
ID
ZDB-PUB-990106-25
Date
1998
Source
General and comparative endocrinology   112: 296-302 (Journal)
Registered Authors
Illing, Nicola
Keywords
Amino Acid Sequence; Animal; Base Sequence; Chickens; Comparative Study; Conserved Sequence; Goldfish; Human; Lizards; Molecular Sequence Data; Polymerase Chain Reaction; Receptors, LHRH; Species Specificity; Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Vertebrates; Xenopus laevis; Zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens
  • Conserved Sequence
  • Goldfish
  • Humans
  • Lizards
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Receptors, LHRH/genetics
  • Receptors, LHRH/metabolism*
  • Species Specificity
  • Vertebrates/metabolism*
  • Xenopus laevis
  • Zebrafish
PubMed
9843635 Full text @ Gen. Comp. Endocrinol.
Abstract
The majority of vertebrates have two or three forms of gonadotropin-releasing hormone (GnRH), which appear to have arisen by successive gene duplication events. This suggests the possibility of concordant gene duplications of the GnRH receptor to produce two or more cognate receptors. Since the extracellular loop 3 (EC3) domain of mammalian GnRH receptors plays a role in distinguishing the different forms of GnRH, we have contemplated that the sequence of this domain will differ significantly in the putative cognate receptors. Degenerate oliognucleotides encoding the sequences of the transmembrane domains preceding and following EC3 were used for PCR amplification of genomic DNA from zebrafish (Brachydanio rerio), goldfish (Carassius auratus), African clawed frog (Xenopus laevis), chicken (Gallus domesticus), and lizard (Agama atra). Isolation and sequencing of specific clones revealed that they fell into three groups. Two of these were most similar to the mammalian pituitary GnRH receptor and were therefore designated Type IA and Type IB. The third form (designated Type II) was most different from the others and was identified in Xenopus, lizard, and human DNA. These findings support the concept of the existence of three distinct GnRH receptors, which have evolved in conjunction with three distinct GnRH ligand classes present in many vertebrates.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping