|ZFIN ID: ZDB-PUB-981208-28|
ß-lactamase as a marker for gene expression in live zebrafish embryos
Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W.
|Source:||Developmental Biology 203: 290-294 (Journal)|
|Registered Authors:||Driever, Wolfgang, Raz, Erez|
|Keywords:||zebrafish; ß-lactamase; dominant marker|
|PubMed:||9808780 Full text @ Dev. Biol.|
Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W. (1998) ß-lactamase as a marker for gene expression in live zebrafish embryos. Developmental Biology. 203:290-294.
ABSTRACTIn this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme catalyzes the hydrolysis of the substrate, thereby disrupting fluorescence resonance energy transfer from the donor to the acceptor dye in the molecule. As a result, a blue fluorescent product is produced and retained specifically in cells within which the enzyme is expressed. beta-Lactamase is therefore suitable for monitoring spatially restricted patterns of gene expression in the early embryo. We suggest that this new reporter system provides a major advancement in sensitivity over the existing methods for monitoring gene expression in vivo during early embryogenesis.
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