ZFIN ID: ZDB-PUB-981208-28
ß-lactamase as a marker for gene expression in live zebrafish embryos
Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W.
Date: 1998
Source: Developmental Biology   203: 290-294 (Journal)
Registered Authors: Driever, Wolfgang, Raz, Erez
Keywords: zebrafish; ß-lactamase; dominant marker
MeSH Terms:
  • Animals
  • Biomarkers*
  • Fluoresceins/metabolism
  • Fluorescence
  • Fluorescent Dyes/metabolism
  • Gene Expression Regulation, Developmental/genetics*
  • Gene Expression Regulation, Enzymologic/genetics
  • Genes, Reporter/genetics
  • Green Fluorescent Proteins
  • Lactams*
  • Luminescent Proteins/genetics
  • Zebrafish/embryology*
  • beta-Lactamases/genetics*
  • beta-Lactams/metabolism
PubMed: 9808780 Full text @ Dev. Biol.
In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme catalyzes the hydrolysis of the substrate, thereby disrupting fluorescence resonance energy transfer from the donor to the acceptor dye in the molecule. As a result, a blue fluorescent product is produced and retained specifically in cells within which the enzyme is expressed. beta-Lactamase is therefore suitable for monitoring spatially restricted patterns of gene expression in the early embryo. We suggest that this new reporter system provides a major advancement in sensitivity over the existing methods for monitoring gene expression in vivo during early embryogenesis.