PUBLICATION
Effects of hirudin (thrombin specific inhibitor) in zebrafish embryos: a developmental role for thrombin
- Authors
- Jagadeeswaran, P., Liu, Y.C., and Eddy, C.A.
- ID
- ZDB-PUB-980105-4
- Date
- 1997
- Source
- Blood cells, molecules & diseases 23(21): 410-414 (Journal)
- Registered Authors
- Jagadeeswaran, Pudur
- Keywords
- hirudin; thrombin; hemostasis; development; zebrafish
- MeSH Terms
-
- Animals
- Antithrombins/pharmacology*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/enzymology*
- Enzyme Activation/drug effects
- Hirudins/administration & dosage
- Hirudins/pharmacology*
- Microinjections
- Thrombin/antagonists & inhibitors*
- Thrombin/physiology*
- Zebrafish
- PubMed
- 9446755 Full text @ Blood Cells Mol. Dis.
Citation
Jagadeeswaran, P., Liu, Y.C., and Eddy, C.A. (1997) Effects of hirudin (thrombin specific inhibitor) in zebrafish embryos: a developmental role for thrombin. Blood cells, molecules & diseases. 23(21):410-414.
Abstract
To address the role of thrombin in early embryogenesis, hirudin, a thrombin specific inhibitor was microinjected into developing zebrafish embryos to inhibit the temporal activity of thrombin during early embryonic development. We found that the fibrin forming activity is inhibited by the presence of hirudin. We also found that hirudin affects development in zebrafish embryos suggesting thrombin's role in early embryogenesis. This ability to inhibit thrombin activity in developing embryos should facilitate studies on identifying signal transduction pathways affected by thrombin during embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping