PUBLICATION

Effects of hirudin (thrombin specific inhibitor) in zebrafish embryos: a developmental role for thrombin

Authors
Jagadeeswaran, P., Liu, Y.C., and Eddy, C.A.
ID
ZDB-PUB-980105-4
Date
1997
Source
Blood cells, molecules & diseases   23(21): 410-414 (Journal)
Registered Authors
Jagadeeswaran, Pudur
Keywords
hirudin; thrombin; hemostasis; development; zebrafish
MeSH Terms
  • Animals
  • Antithrombins/pharmacology*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/enzymology*
  • Enzyme Activation/drug effects
  • Hirudins/administration & dosage
  • Hirudins/pharmacology*
  • Microinjections
  • Thrombin/antagonists & inhibitors*
  • Thrombin/physiology*
  • Zebrafish
PubMed
9446755 Full text @ Blood Cells Mol. Dis.
Abstract
To address the role of thrombin in early embryogenesis, hirudin, a thrombin specific inhibitor was microinjected into developing zebrafish embryos to inhibit the temporal activity of thrombin during early embryonic development. We found that the fibrin forming activity is inhibited by the presence of hirudin. We also found that hirudin affects development in zebrafish embryos suggesting thrombin's role in early embryogenesis. This ability to inhibit thrombin activity in developing embryos should facilitate studies on identifying signal transduction pathways affected by thrombin during embryogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping