PUBLICATION
A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula
- Authors
- Fürthauer, M., Thisse, C., and Thisse, B.
- ID
- ZDB-PUB-971203-8
- Date
- 1997
- Source
- Development (Cambridge, England) 124(21): 4253-4264 (Journal)
- Registered Authors
- Fürthauer, Maximilian, Thisse, Bernard, Thisse, Christine
- Keywords
- fgf-8; Spemann's organizer; gastrulation; dorsoventral patterning; convergence; BMP2; BMP4; Chordin; Noggin; Goosecoid
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Blastocyst/physiology
- Body Patterning/genetics*
- Bone Morphogenetic Protein 2
- Bone Morphogenetic Protein 4
- Bone Morphogenetic Proteins/genetics
- Bone Morphogenetic Proteins/metabolism
- Cloning, Molecular
- Embryo, Nonmammalian
- Embryonic Induction/genetics
- Fibroblast Growth Factor 8
- Fibroblast Growth Factors*
- Gastrula/physiology*
- Gene Expression Regulation, Developmental
- Growth Substances/physiology*
- Mesencephalon/embryology
- Molecular Sequence Data
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Transcription, Genetic
- Transforming Growth Factor beta*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins
- PubMed
- 9334274 Full text @ Development
Citation
Fürthauer, M., Thisse, C., and Thisse, B. (1997) A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula. Development (Cambridge, England). 124(21):4253-4264.
Abstract
Signals released from Spemann's organizer, together with ventralizing factors such as BMPs, are necessary to pattern the dorsoventral axis of the vertebrate embryo. We report that a member of the FGF family, fgf-8, not secreted by the axial mesoderm but expressed in a dorsoventral gradient at the margin of the zebrafish gastrula, also contributes to the establishment of the dorsoventral axis of the embryo. Ectopic expression of FGF-8 leads to the expansion of dorsolateral derivatives at the expense of ventral and posterior domains. Moreover, FGF-8 displays some organizer properties as it induces the formation of a partial secondary axis in the absence of factors released from Spemann's organizer territory. Analysis of its interaction with the ventralizing factors, BMPs, reveals that overexpression of FGF-8 inhibits the expression of these factors in the ventral part of the embryo as early as blastula stage, suggesting that FGF-8 acts upstream of BMP2 and BMP4. We conclude that FGF-8 is involved in defining dorsoventral identity and is an important organizing factor responsible for specification of mesodermal and ectodermal dorsolateral territories of the zebrafish gastrula.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping