PUBLICATION
Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A
- Authors
- Slusarski, D.C., Yang-Snyder, J., Busa, W.B., and Moon, R.T.
- ID
- ZDB-PUB-970918-25
- Date
- 1997
- Source
- Developmental Biology 182(1): 114-120 (Journal)
- Registered Authors
- Moon, Randall T., Slusarski, Diane C.
- Keywords
- none
- MeSH Terms
-
- Animals
- Blastocyst/physiology*
- Calcium/metabolism*
- Embryo, Nonmammalian/physiology
- Proto-Oncogene Proteins/biosynthesis
- Proto-Oncogene Proteins/physiology*
- Receptor, Serotonin, 5-HT2C
- Receptors, Serotonin/biosynthesis
- Receptors, Serotonin/physiology*
- Signal Transduction*
- Vertebrates
- Wnt Proteins
- Zebrafish
- PubMed
- 9073455 Full text @ Dev. Biol.
Citation
Slusarski, D.C., Yang-Snyder, J., Busa, W.B., and Moon, R.T. (1997) Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A. Developmental Biology. 182(1):114-120.
Abstract
Wnt genes encode secreted proteins which are implicated in receptor-mediated cell-cell signaling events important in embryogenesis, but the second messenger systems modulated by Wnts have not been identified. We report that ectopic expression of Xwnt-5A in zebrafish embryos enhances the frequency of intracellular Ca2+ transients in the enveloping layer of the blastodisc, whereas Xwnt-8 does not. These transients are independent of extracellular Ca2+. Consistent with the observed Ca2+ transients playing a role in responses of embryos to Xwnt-5A, we report that the ligand-activated serotonin type 1C receptor, which stimulates PI cycle activity and Ca2+ signaling independent of Wnts, phenocopies embryonic responses to Xwnt-5A. These results suggest that intercellular signaling by a subset of vertebrate Wnts involves modulation of a intracellular Ca2+ signaling pathway, which may arise from phosphatidylinositol cycle activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping