PUBLICATION

Structure and transcription of the gene for translation elongation factor 1 subunit alpha of zebrafish (Danio rerio)

Authors
Gao, D.Y., Li, Z.F., Murphy, T., and Sauerbier, W.
ID
ZDB-PUB-970320-1
Date
1997
Source
Biochim. Biophys. Acta Gene Struct. Exp.   1350(1): 1-5 (Journal)
Registered Authors
Li, Zheng, Sauerbier, W.
Keywords
translation elongation factor 1a; gene structure; (zebrafish)
MeSH Terms
  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • DNA Primers
  • Embryo, Nonmammalian/physiology
  • Exons
  • Fertilization
  • Gene Expression Regulation, Developmental
  • Genomic Library
  • Haploidy
  • Introns
  • Macromolecular Substances
  • Molecular Sequence Data
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors/biosynthesis*
  • Peptide Elongation Factors/genetics*
  • Polymerase Chain Reaction
  • Pseudogenes
  • Restriction Mapping
  • Transcription, Genetic*
  • Zebrafish/genetics*
PubMed
9003448 Full text @ Biochim. Biophys. Acta Gene Struct. Exp.
Abstract
The zebrafish gene for translation elongation factor 1 alpha (EF1 alpha) was isolated from a phage Lambda genomic library and sequence and structure determined. One gene copy of EF1 alpha per haploid set of chromosomes was found and no processed pseudogenes. A highly active promoter region was localized to a 277 bp PstI/PvuII fragment beginning 240 bp upstream from the tsp, but no transcription enhancing, or silencing activity was observed within 1 kbp upstream, or downstream from the promoter. Expression of EF1 alpha appears to be developmentally regulated.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping