PUBLICATION
Multiple elements may be used for regulation of the GAP-43 gene in different cell-types
- Authors
- Reinhard, E. and Skene, J.H.
- ID
- ZDB-PUB-970102-15
- Date
- 1992
- Source
- Perspectives on developmental neurobiology 1: 29-37 (Journal)
- Registered Authors
- Reinhard, Eva, Skene, J.H.P.
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Axons/metabolism
- Dopa Decarboxylase/biosynthesis
- Dopa Decarboxylase/genetics
- Enhancer Elements, Genetic
- Epidermis/embryology
- GAP-43 Protein
- Gastrula/physiology
- Gene Expression Regulation*
- Genes
- Membrane Glycoproteins/genetics*
- Nerve Tissue Proteins/genetics*
- Nervous System/embryology
- Organ Specificity
- Promoter Regions, Genetic
- Rats
- Recombinant Fusion Proteins/biosynthesis
- Regulatory Sequences, Nucleic Acid*
- Signal Transduction
- Species Specificity
- Transcription Factors/metabolism
- Zebrafish
- PubMed
- 1345682
Citation
Reinhard, E. and Skene, J.H. (1992) Multiple elements may be used for regulation of the GAP-43 gene in different cell-types. Perspectives on developmental neurobiology. 1:29-37.
Abstract
Recent evidence suggests that GAP-43 expression is not restricted to the nervous system, but may also occur outside the neural cell lineage. Two distinct patterns of GAP-43 regulation can therefore be distinguished. The first is the regulation of GAP-43 expression in multiple cell-types, and the second is the gene's temporal modulation within one specific cell-type. The latter type is well documented for neurons, where GAP-43 regulation is regulated in a fashion that is dependent on axon integrity. Results from partial analysis of the GAP-43 promoter/enhancer region indicate that at least some of these aspects of GAP-43 gene regulation may be accounted for by distinct cis-acting elements. For example, the expression of a rat GAP-43 promoter fusion gene in epidermal cells of transgenic zebrafish is dependent on an enhancer element, that is clearly distinct from the minimal neural- specific promoter. Characterization of specific GAP-43 regulatory elements responsible for particular aspects of its regulation may provide insight to signal pathways also utilized by other genes during development. Ultimately, a better understanding of the molecular events during development could help to define more precisely the complex sequences necessary for the establishment of an intact organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping