PUBLICATION
The RNA subunit of ribonuclease P from the zebrafish, Danio rerio
- Authors
- Eder, P.S., Srinivasan, A., Fishman, M.C., and Altman, S.
- ID
- ZDB-PUB-961107-3
- Date
- 1996
- Source
- The Journal of biological chemistry 271(35): 21031-21036 (Journal)
- Registered Authors
- Fishman, Mark C., Srinivasan, Ashok
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Cloning, Molecular
- DNA
- Endoribonucleases/genetics*
- Humans
- Molecular Sequence Data
- Nucleic Acid Conformation
- RNA/chemistry
- RNA/genetics*
- RNA, Catalytic/genetics*
- Ribonuclease P
- Sequence Homology, Nucleic Acid
- Zebrafish/genetics*
- PubMed
- 8702867 Full text @ J. Biol. Chem.
Citation
Eder, P.S., Srinivasan, A., Fishman, M.C., and Altman, S. (1996) The RNA subunit of ribonuclease P from the zebrafish, Danio rerio. The Journal of biological chemistry. 271(35):21031-21036.
Abstract
A simple strategy has been devised to identify the gene encoding the RNA subunit of RNase P from the zebrafish, Danio rerio. The sequence obtained by amplification of genomic DNA with primers based on sequences common to two other vertebrates was confirmed by reverse transcription and amplification of RNA from a partially purified preparation of the holoenzyme. The 5' and 3' ends were determined by cyclizing the RNA, followed by reverse transcription and sequencing across the ligated RNA junction. The zebrafish sequence is 63% identical to that of Xenopus laevis nuclear RNase P RNA and 69% identical to the human RNase P RNA. A consensus secondary structure was constructed based on these nucleotide identities and on the many compensatory base changes in several regions among these three RNAs. The strategy used to obtain the zebrafish sequence should be useful in deriving analogous gene sequences from diverse classes of eukaryotes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping