PUBLICATION

Primary structure, developmentally regulated expression and potential duplication of the zebrafish homeobox gene ZF-21

Authors
Njolstad, P.R., Molven, A., Hordvik, I., Apold, J., and Fjose, A.
ID
ZDB-PUB-961014-838
Date
1988
Source
Nucleic acids research   16(19): 9097-9111 (Journal)
Registered Authors
Fjose, Anders, Molven, Anders
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA/genetics
  • Fishes/genetics*
  • Gene Expression Regulation
  • Genes, Homeobox*
  • Molecular Sequence Data
  • Multigene Family
  • RNA, Messenger/genetics
  • Restriction Mapping
PubMed
2902580 Full text @ Nucleic Acids Res.
Abstract
We report the molecular cloning and characterization of a cDNA derived from a zebrafish gene (ZF-21) related to the mouse homeobox containing gene Hox2.1. Interesting information about the differential conservation of various domains was gained from comparisons between the putative protein sequences from ZF-21 (275 amino acids) and Hox2.1 (279 aa). A separate DNA binding domain including the ZF-21 homeodomain and 36 additional flanking residues is completely identical to the C- terminal part of Hox2.1. As a consequence, these two mouse and zebrafish proteins must have identical DNA binding properties. A lower level of sequence identity between the N- terminal coding regions of ZF-21 and Hox2.1 reduces the total protein homology to 81%. However, short stretches of perfect homology in these N-terminals suggests that the essential biochemical functions are the same. As expected for true homologues, the ZF-21 and Hox2.1 genes also share extensive similarities with respect to non-coding sequences and temporal expression during embryogenesis. The finding of a potential ZF- 21 duplication is discussed in relation to functional and evolutionary aspects of vertebrate homeobox genes.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping