PUBLICATION

Genotype-specific modifiers of transgene methylation and expression in the zebrafish, Danio rerio

Authors
Martin, C.C. and McGowan, R.
ID
ZDB-PUB-961014-736
Date
1995
Source
Gen. Res.   65: 21-28 (Journal)
Registered Authors
Martin, C. Cristofre, McGowan, Ross
Keywords
none
MeSH Terms
  • 5-Methylcytosine
  • Animals
  • Chloramphenicol O-Acetyltransferase/genetics
  • Cytosine/analogs & derivatives*
  • Cytosine/analysis
  • Female
  • Gene Expression Regulation*
  • Genes, Reporter
  • Genes, Synthetic*
  • Genotype
  • Male
  • Methylation
  • Mice
  • Mice, Transgenic
  • Temperature
  • Zebrafish/genetics*
PubMed
7750743 Full text @ Gen. Res.
Abstract
Previous reports involving mammalian systems, particularly mice, have demonstrated the existence of cis- and trans-acting modifiers of transgene methylation. These modifiers are thought to be important in dominance modification, genome imprinting and cellular expression mosaicism. Their potential role in the penetrance and severity of many complex human diseases could be of even greater significance. In the present investigation we demonstrate that modifiers that act in a similar fashion to those identified in mice also exist in a non-mammalian vertebrate, the zebrafish Danio rerio. We also provide evidence that the transgene methylation pattern may be influenced by the sex of the individual and environmental modulators such as temperature and sodium butyrate. These data support the theory that this type of dominance modification is mechanistically similar to position effect variegation in Drosophila. Furthermore, these data suggest evolutionary conservation of the modifiers, at least within vertebrates, and imply that they and their actions are important in normal vertebrate development.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping