PUBLICATION
Fish embryo cell cultures for derivation of stem cells and transgenic chimeras
- Authors
- Collodi, P., Kamei, Y., Sharps, A., Weber, D., and Barnes, D.
- ID
- ZDB-PUB-961014-180
- Date
- 1992
- Source
- Molecular marine biology and biotechnology 1: 257-265 (Journal)
- Registered Authors
- Barnes, David W., Collodi, Paul, Sharps, Angela, Weber, Dan
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/embryology*
- Animals, Genetically Modified/genetics
- Blastocyst/cytology
- Blastocyst/enzymology
- Cells, Cultured
- Chimera*
- Culture Media, Serum-Free
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/enzymology
- Female
- Flow Cytometry
- Gene Expression
- Haploidy
- Karyotyping
- Male
- Plasmids/physiology
- Promoter Regions, Genetic/physiology
- Stem Cell Transplantation*
- Transfection
- Trout/embryology*
- Trout/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- beta-Galactosidase/biosynthesis
- PubMed
- 1308815
Citation
Collodi, P., Kamei, Y., Sharps, A., Weber, D., and Barnes, D. (1992) Fish embryo cell cultures for derivation of stem cells and transgenic chimeras. Molecular marine biology and biotechnology. 1:257-265.
Abstract
It has been demonstrated in mammalian systems that techniques using embryonal stem cells provide advantages over conventional injection of DNA into embryos for generation of transgenic animals. We employed cell culture approaches in an attempt to develop this technology for fish transgenesis. Using a trout embryo-derived mitogenic preparation in a specialized culture medium, we initiated replication of zebrafish blastula- derived cell cultures and expressed marker genes introduced into the cells by plasmid transfection. Reintroduction of cells from the cultures into blastula-stage embryos indicated that the cultured cells survived and may contribute to the developing organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping