Glucocorticoid receptor activation reduces food intake independent of hyperglycemia in zebrafish
- Nipu, N., Antomagesh, F., Faught, E., Vijayan, M.M.
- Scientific Reports 12: 15677 (Journal)
- Registered Authors
- Faught, Erin, Vijayan, Mathilakath
- MeSH Terms
- AMP-Activated Protein Kinases/metabolism
- Amino Acids
- Proto-Oncogene Proteins c-akt
- Receptors, Glucocorticoid*/metabolism
- TOR Serine-Threonine Kinases
- 36127383 Full text @ Sci. Rep.
Nipu, N., Antomagesh, F., Faught, E., Vijayan, M.M. (2022) Glucocorticoid receptor activation reduces food intake independent of hyperglycemia in zebrafish. Scientific Reports. 12:15677.
Chronic cortisol exposure suppresses food intake in fish, but the central mechanism(s) involved in appetite regulation are unclear. Stress and the associated increase in cortisol levels increase hepatic gluconeogenesis, leading to hyperglycemia. As hyperglycemia causes a reduction in food intake, we tested the hypothesis that cortisol-induced hyperglycemia suppresses feeding in zebrafish (Danio rerio). We first established that stress-independent hyperglycemia suppressed food intake, and this corresponded with a reduction in the phosphorylation of the nutrient sensor, AMP-activated protein kinase (AMPK) in the brain. Chronic cortisol exposure also led to hyperglycemia and reduced food intake, but the mechanisms were distinct. In cortisol-exposed fish, there were no changes in brain glucose uptake or AMPK phosphorylation. Also, the phosphorylation of Akt and mTOR was reduced along with an increase in redd1, suggesting an enhanced capacity for proteolysis. Loss of the glucocorticoid receptor did not rescue cortisol-mediated feeding suppression but did increase glucose uptake and abolished the changes seen in mTOR phosphorylation and redd1 transcript abundance. Taken together, our results indicate that GR activation enhances brain proteolysis, and the associated amino acids levels, and not hyperglycemia, maybe a key mediator of the feeding suppression in response to chronic cortisol stimulation in zebrafish.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes