PUBLICATION
Longitudinal investigation of a xenograft tumor zebrafish model using polarization-sensitive optical coherence tomography
- Authors
- Lichtenegger, A., Tamaoki, J., Licandro, R., Mori, T., Mukherjee, P., Bian, L., Greutter, L., Makita, S., Wöhrer, A., Matsusaka, S., Kobayashi, M., Baumann, B., Yasuno, Y.
- ID
- ZDB-PUB-220915-1
- Date
- 2022
- Source
- Scientific Reports 12: 15381 (Journal)
- Registered Authors
- Kobayashi, Makoto, Tamaoki, Junya
- Keywords
- none
- MeSH Terms
-
- Animals
- Breast Neoplasms*
- Disease Models, Animal
- Female
- Heterografts
- Humans
- Longitudinal Studies
- Tomography, Optical Coherence*/methods
- Zebrafish
- PubMed
- 36100620 Full text @ Sci. Rep.
Citation
Lichtenegger, A., Tamaoki, J., Licandro, R., Mori, T., Mukherjee, P., Bian, L., Greutter, L., Makita, S., Wöhrer, A., Matsusaka, S., Kobayashi, M., Baumann, B., Yasuno, Y. (2022) Longitudinal investigation of a xenograft tumor zebrafish model using polarization-sensitive optical coherence tomography. Scientific Reports. 12:15381.
Abstract
Breast cancer is a leading cause of death in female patients worldwide. Further research is needed to get a deeper insight into the mechanisms involved in the development of this devastating disease and to find new therapy strategies. The zebrafish is an established animal model, especially in the field of oncology, which has shown to be a promising candidate for pre-clinical research and precision-based medicine. To investigate cancer growth in vivo in zebrafish, one approach is to explore xenograft tumor models. In this article, we present the investigation of a juvenile xenograft zebrafish model using a Jones matrix optical coherence tomography (JM-OCT) prototype. Immunosuppressed wild-type fish at 1-month post-fertilization were injected with human breast cancer cells and control animals with phosphate buffered saline in the tail musculature. In a longitudinal study, the scatter, polarization, and vasculature changes over time were investigated and quantified in control versus tumor injected animals. A significant decrease in birefringence and an increase in scattering signal was detected in tumor injected zebrafish in comparison to the control once. This work shows the potential of JM-OCT as a non-invasive, label-free, three-dimensional, high-resolution, and tissue-specific imaging tool in pre-clinical cancer research based on juvenile zebrafish models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping