PUBLICATION

Both prokaryotes and eukaryotes produce an immune response against plasmids with 5'-GTTTGTT-3'

Authors
Li, N., Jiang, D., He, L., Yue, Y., Zhang, Q., Wang, S., Zhang, Y., Wei, Y., Zhao, Q.
ID
ZDB-PUB-220613-1
Date
2022
Source
Cell & Bioscience   12: 87 (Journal)
Registered Authors
Yue, Yunyun, Zhao, Qingshun
Keywords
5ʹ-GTTTGTT-3ʹ, Core sequence, Defense response, Eukaryotic cell, Foreign plasmid, ISG15, Innate immune memory, Prokaryotic cell, Transformation efficiency
Datasets
GEO:GSE165422
MeSH Terms
none
PubMed
35690839 Full text @ Cell Biosci.
Abstract
In the evolutionary "arms race" from prokaryotes to eukaryotes, some memories of foreign DNA have been conserved for defensive purposes. Shortly after invasion by the plasmid, pEGFP-N1, the conserved the defense gene, isg15, was activated in the zebrafish zygote and in mammalian cells. Based on the sequence similarity, we found three virus-derived sequences in pEGFP-N1 which share the 5'-GTTTGTT-3' core sequence, an epigenetic factor leading to increased expression of isg15. Mutation of the core sequence greatly reduces the degradation rate of the plasmid in E. coli cells or zebrafish embryos. We conclude that a conserved defense response, common to both eukaryotic and prokaryotic cells, allows identification and degradation of plasmids containing 5'-GTTTGTT-3'.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping