PUBLICATION
Multimodal Characterization of Seizures in Zebrafish Larvae
- Authors
- Turrini, L., Sorelli, M., de Vito, G., Credi, C., Tiso, N., Vanzi, F., Pavone, F.S.
- ID
- ZDB-PUB-220530-8
- Date
- 2022
- Source
- Biomedicines 10(5): (Journal)
- Registered Authors
- Tiso, Natascia, Turrini, Lapo, Vanzi, Francesco
- Keywords
- behavior, calcium imaging, epilepsy, functional connectivity, light-sheet microscopy, seizure, tracking, two-photon, zebrafish
- MeSH Terms
- none
- PubMed
- 35625689 Full text @ Biomedicines
Citation
Turrini, L., Sorelli, M., de Vito, G., Credi, C., Tiso, N., Vanzi, F., Pavone, F.S. (2022) Multimodal Characterization of Seizures in Zebrafish Larvae. Biomedicines. 10(5).
Abstract
Epilepsy accounts for a significant proportion of the world's disease burden. Indeed, many research efforts are produced both to investigate the basic mechanism ruling its genesis and to find more effective therapies. In this framework, the use of zebrafish larvae, owing to their peculiar features, offers a great opportunity. Here, we employ transgenic zebrafish larvae expressing GCaMP6s in all neurons to characterize functional alterations occurring during seizures induced by pentylenetetrazole. Using a custom two-photon light-sheet microscope, we perform fast volumetric functional imaging of the entire larval brain, investigating how different brain regions contribute to seizure onset and propagation. Moreover, employing a custom behavioral tracking system, we outline the progressive alteration of larval swim kinematics, resulting from different grades of seizures. Collectively, our results show that the epileptic larval brain undergoes transitions between diverse neuronal activity regimes. Moreover, we observe that different brain regions are progressively recruited into the generation of seizures of diverse severity. We demonstrate that midbrain regions exhibit highest susceptibility to the convulsant effects and that, during periods preceding abrupt hypersynchronous paroxysmal activity, they show a consistent increase in functional connectivity. These aspects, coupled with the hub-like role that these regions exert, represent important cues in their identification as epileptogenic hubs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping