PUBLICATION

Coordinated activities of Myosin Vb isoforms and mTOR signaling regulate epithelial cell morphology during development

Authors
Gupta, K., Mukherjee, S., Sen, S., Sonawane, M.
ID
ZDB-PUB-220318-14
Date
2022
Source
Development (Cambridge, England)   149(6): (Journal)
Registered Authors
Sonawane, Mahendra
Keywords
Cell size regulation, Compensatory cell growth, Epidermis, Myosin Vb, Tissue homeostasis, Zebrafish, mTOR signaling
MeSH Terms
  • Animals
  • Epithelial Cells/metabolism
  • Myosin Type V*/metabolism
  • Protein Isoforms/metabolism
  • TOR Serine-Threonine Kinases/metabolism
  • Zebrafish/metabolism
  • rab GTP-Binding Proteins/metabolism
PubMed
35299238 Full text @ Development
Abstract
The maintenance of epithelial architecture necessitates tight regulation of cell size and shape. However, mechanisms underlying epithelial cell size regulation remain poorly understood. We show that the interaction of Myosin Vb with Rab11 prevents the accumulation of apically derived endosomes to maintain cell-size, whereas that with Rab10 regulates vesicular transport from the trans-Golgi. These interactions are required for the fine-tuning of the epithelial cell morphology during zebrafish development. Furthermore, the compensatory cell growth upon cell-proliferation inhibition involves a preferential expansion of the apical domain, leading to flatter epithelial cells, an efficient strategy to cover the surface with fewer cells. This apical domain growth requires post-trans-Golgi transport mediated by the Rab10-interacting Myosin Vb isoform, downstream of the mTOR-Fatty Acid Synthase (FASN) axis. Changes in trans-Golgi morphology indicate that the Golgi synchronizes mTOR-FASN-regulated biosynthetic input and Myosin Vb-Rab10 dependent output. Our study unravels the mechanism of polarized growth in epithelial cells and delineates functions of Myosin Vb isoforms in cell size regulation during development.
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