PUBLICATION
5' Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
- Authors
- Chen, L., Xu, W., Liu, K., Jiang, Z., Han, Y., Jin, H., Zhang, L., Shen, W., Jia, S., Sun, Q., Meng, A.
- ID
- ZDB-PUB-220303-35
- Date
- 2021
- Source
- Science advances 7: eabh0494 (Journal)
- Registered Authors
- Jia, Shunji, Meng, Anming
- Keywords
- none
- Datasets
- GEO:GSE181684, GEO:GSE147253, GEO:GSE181683
- MeSH Terms
- none
- PubMed
- 34797706 Full text @ Sci Adv
Citation
Chen, L., Xu, W., Liu, K., Jiang, Z., Han, Y., Jin, H., Zhang, L., Shen, W., Jia, S., Sun, Q., Meng, A. (2021) 5' Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos. Science advances. 7:eabh0494.
Abstract
5′tRFls are small transfer RNA (tRNA) fragments derived from 5′ half of mature tRNAs. However, it is unknown whether 5′tRFls could feed back to regulate tRNA biogenesis. Here, we show that 5′tRFlGly/GCC and 5′tRFlGlu/CTC function to promote transcription of corresponding tRNA genes and are essential for vertebrate early embryogenesis. During zebrafish embryogenesis, dynamics of 5′tRFlGly/GCC and 5′tRFlGlu/CTC levels correlates with that of tRNAGly/GCC and tRNAGlu/CTC levels. Morpholino-mediated knockdown of 5′tRFlGly/GCC or 5′tRFlGlu/CTC down-regulates tRNAGly/GCC or tRNAGlu/CTC levels, respectively, and causes embryonic lethality that is efficiently rescued by coinjection of properly refolded corresponding tRNA. In zebrafish embryos, tRNA:DNA and 5′tRFl:DNA hybrids commonly exist on the template strand of tRNA genes. Mechanistically, unstable 5′tRFl:DNA hybrid may prevent the formation of transcriptionally inhibitory stable tRNA:DNA hybrids on the same tRNA loci so as to facilitate tRNA gene transcription. The uncovered mechanism may be implicated in other physiological and pathological processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping