PUBLICATION
An early Shh-H2O2 reciprocal regulatory interaction controls the regenerative program during zebrafish fin regeneration
- Authors
- Thauvin, M., de Sousa, R.M., Alves, M., Volovitch, M., Vriz, S., Rampon, C.
- ID
- ZDB-PUB-220203-6
- Date
- 2022
- Source
- Journal of Cell Science 135(6): (Journal)
- Registered Authors
- Rampon, Christine, Vriz, Sophie
- Keywords
- Fin regeneration, Hedgehog signaling, Hydrogen peroxide, Redox, Regeneration, Sonic Hedgehog, Zebrafish
- MeSH Terms
-
- Animals
- Hedgehog Proteins*/metabolism
- Hydrogen Peroxide/pharmacology
- Reactive Oxygen Species
- Wound Healing
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 35107164 Full text @ J. Cell Sci.
Citation
Thauvin, M., de Sousa, R.M., Alves, M., Volovitch, M., Vriz, S., Rampon, C. (2022) An early Shh-H2O2 reciprocal regulatory interaction controls the regenerative program during zebrafish fin regeneration. Journal of Cell Science. 135(6):.
Abstract
Reactive oxygen species (ROS), originally classified as toxic molecules, have attracted increasing interest given their actions in cell signaling. Hydrogen peroxide (H2O2), the major ROS produced by cells, acts as a second messenger to modify redox-sensitive proteins or lipids. After caudal fin amputation, tight spatiotemporal regulation of ROS is required first for wound healing and later to initiate the regenerative program. However, the mechanisms carrying out this sustained ROS production and their integration with signaling pathways are still poorly understood. We focused on the early dialog between H2O2 and Sonic Hedgehog (Shh) during fin regeneration. We demonstrate that H2O2 controls Shh expression and that Shh in turn regulates the H2O2 level via a canonical pathway. Moreover, the means of this tight reciprocal control change during the successive phases of the regenerative program. Dysregulation of the Hedgehog pathway has been implicated in several developmental syndromes, diabetes and cancer. These data support the existence of an early positive crosstalk between Shh and H2O2 that might be more generally involved in various processes paving the way to improve regenerative processes, particularly in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping