PUBLICATION
Mandipropamid as a chemical inducer of proximity for in vivo applications
- Authors
- Ziegler, M.J., Yserentant, K., Dunsing, V., Middel, V., Gralak, A.J., Pakari, K., Bargstedt, J., Kern, C., Petrich, A., Chiantia, S., Strähle, U., Herten, D.P., Wombacher, R.
- ID
- ZDB-PUB-211224-5
- Date
- 2021
- Source
- Nature Chemical Biology 18(1): 64-69 (Journal)
- Registered Authors
- Middel, Volker, Strähle, Uwe
- Keywords
- none
- MeSH Terms
-
- Abscisic Acid/metabolism
- Amides/pharmacology*
- Animals
- Carboxylic Acids/pharmacology*
- Dimerization
- Fungicides, Industrial/pharmacology*
- Zebrafish/embryology
- PubMed
- 34934192 Full text @ Nat. Chem. Biol.
Citation
Ziegler, M.J., Yserentant, K., Dunsing, V., Middel, V., Gralak, A.J., Pakari, K., Bargstedt, J., Kern, C., Petrich, A., Chiantia, S., Strähle, U., Herten, D.P., Wombacher, R. (2021) Mandipropamid as a chemical inducer of proximity for in vivo applications. Nature Chemical Biology. 18(1):64-69.
Abstract
Direct control of protein interactions by chemically induced protein proximity holds great potential for both cell and synthetic biology as well as therapeutic applications. Low toxicity, orthogonality and excellent cell permeability are important criteria for chemical inducers of proximity (CIPs), in particular for in vivo applications. Here, we present the use of the agrochemical mandipropamid (Mandi) as a highly efficient CIP in cell culture systems and living organisms. Mandi specifically induces complex formation between a sixfold mutant of the plant hormone receptor pyrabactin resistance 1 (PYR1) and abscisic acid insensitive (ABI). It is orthogonal to other plant hormone-based CIPs and rapamycin-based CIP systems. We demonstrate the applicability of the Mandi system for rapid and efficient protein translocation in mammalian cells and zebrafish embryos, protein network shuttling and manipulation of endogenous proteins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping