PUBLICATION
Formimidoyltransferase cyclodeaminase prevents the starvation-induced liver hepatomegaly and dysfunction through downregulating mTORC1
- Authors
- Zhang, W., Wu, C., Ni, R., Yang, Q., Luo, L., He, J.
- ID
- ZDB-PUB-211224-26
- Date
- 2021
- Source
- PLoS Genetics 17: e1009980 (Journal)
- Registered Authors
- Luo, Lingfei
- Keywords
- none
- MeSH Terms
-
- Ammonia-Lyases/genetics*
- Animals
- Disease Models, Animal
- Glutamate Formimidoyltransferase/genetics*
- Hepatocytes/metabolism
- Hepatocytes/pathology
- Hepatomegaly/genetics*
- Hepatomegaly/metabolism
- Hepatomegaly/pathology
- Humans
- Liver/metabolism*
- Liver/pathology
- Mechanistic Target of Rapamycin Complex 1/genetics
- Multifunctional Enzymes/genetics*
- Multiprotein Complexes/genetics
- Mutation/genetics
- Phosphorylation
- Ribosomal Protein S6/genetics*
- Signal Transduction/genetics
- Starvation/genetics
- Starvation/metabolism
- Starvation/pathology
- Zebrafish/genetics
- PubMed
- 34941873 Full text @ PLoS Genet.
Citation
Zhang, W., Wu, C., Ni, R., Yang, Q., Luo, L., He, J. (2021) Formimidoyltransferase cyclodeaminase prevents the starvation-induced liver hepatomegaly and dysfunction through downregulating mTORC1. PLoS Genetics. 17:e1009980.
Abstract
The liver is a crucial center in the regulation of energy homeostasis under starvation. Although downregulation of mammalian target of rapamycin complex 1 (mTORC1) has been reported to play pivotal roles in the starvation responses, the underpinning mechanisms in particular upstream factors that downregulate mTORC1 remain largely unknown. To identify genetic variants that cause liver energy disorders during starvation, we conduct a zebrafish forward genetic screen. We identify a liver hulk (lvh) mutant with normal liver under feeding, but exhibiting liver hypertrophy under fasting. The hepatomegaly in lvh is caused by enlarged hepatocyte size and leads to liver dysfunction as well as limited tolerance to starvation. Positional cloning reveals that lvh phenotypes are caused by mutation in the ftcd gene, which encodes the formimidoyltransferase cyclodeaminase (FTCD). Further studies show that in response to starvation, the phosphorylated ribosomal S6 protein (p-RS6), a downstream effector of mTORC1, becomes downregulated in the wild-type liver, but remains at high level in lvh. Inhibition of mTORC1 by rapamycin rescues the hepatomegaly and liver dysfunction of lvh. Thus, we characterize the roles of FTCD in starvation response, which acts as an important upstream factor to downregulate mTORC1, thus preventing liver hypertrophy and dysfunction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping