PUBLICATION
A Swimming-based Assay to Determine the Exercise Capacity of Adult Zebrafish Cardiomyopathy Models
- Authors
- Ma, X., Xu, X.
- ID
- ZDB-PUB-210831-9
- Date
- 2021
- Source
- Bio-protocol 11: e4114 (Journal)
- Registered Authors
- Xu, Xiaolei
- Keywords
- Adult Zebrafish, Cardiomyopathy Model, Critical Swimming Speed, Doxorubicin-induced Cardiomyopathy, Exercise Capacity, Exercise Intolerance, Heart Failure, Swimming Tunnel
- MeSH Terms
- none
- PubMed
- 34458408 Full text @ Bio Protoc
Citation
Ma, X., Xu, X. (2021) A Swimming-based Assay to Determine the Exercise Capacity of Adult Zebrafish Cardiomyopathy Models. Bio-protocol. 11:e4114.
Abstract
Exercise capacity, measured by treadmill in humans and other mammals, is an important diagnostic and prognostic index for patients with cardiomyopathy and heart failure. The adult zebrafish is increasingly used as a vertebrate model to study human cardiomyopathy due to its conserved cardiovascular physiology, convenience for genetic manipulation, and amenability to high-throughput genetic and compound screening. Owing to the small size of its body and heart, new phenotyping assays are needed to unveil phenotypic traits of cardiomyopathy in adult zebrafish. Here, we describe a swimming-based functional assay that measures exercise capacity in an adult zebrafish doxorubicin-induced cardiomyopathy model. This protocol can be applied to any adult zebrafish model of acquired or inherited cardiomyopathy and potentially to other cardiovascular diseases. Graphic abstract: Clinical relevance of the swimming-based phenotyping assay in adult zebrafish cardiomyopathy models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping