PUBLICATION
Shuttle Box Assay as an Associative Learning Tool for Cognitive Assessment in Learning and Memory Studies using Adult Zebrafish
- Authors
- Hentig, J., Cloghessy, K., Hyde, D.R.
- ID
- ZDB-PUB-210728-33
- Date
- 2021
- Source
- Journal of visualized experiments : JoVE (173): (Journal)
- Registered Authors
- Hyde, David R.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cognition
- Conditioning, Classical*
- Memory, Short-Term
- Neuropsychological Tests
- Zebrafish*
- PubMed
- 34309605 Full text @ J. Vis. Exp.
Citation
Hentig, J., Cloghessy, K., Hyde, D.R. (2021) Shuttle Box Assay as an Associative Learning Tool for Cognitive Assessment in Learning and Memory Studies using Adult Zebrafish. Journal of visualized experiments : JoVE. (173):.
Abstract
Cognitive deficits, including impaired learning and memory, are a primary symptom of various developmental and age-related neurodegenerative diseases and traumatic brain injury (TBI). Zebrafish are an important neuroscience model due to their transparency during development and robust regenerative capabilities following neurotrauma. While various cognitive tests exist in zebrafish, most of the cognitive assessments that are rapid examine non-associative learning. At the same time, associative-learning assays often require multiple days or weeks. Here, we describe a rapid associative-learning test that utilizes an adverse stimulus (electric shock) and requires minimal preparation time. The shuttle box assay, presented here, is simple, ideal for novice investigators, and requires minimal equipment. We demonstrate that, following TBI, this shuttle box test reproducibly assesses cognitive deficit and recovery from young to old zebrafish. Additionally, the assay is adaptable to examine either immediate or delayed memory. We demonstrate that both a single TBI and repeated TBI events negatively affect learning and immediate memory but not delayed memory. We, therefore, conclude that the shuttle box assay reproducibly tracks the progression and recovery of cognitive impairment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping