PUBLICATION
Application of tumor necrosis factor α mutant zebrafish in Mycobacterium marinum infection
- Authors
- Xie, Lingling, Wang, Fenghua, Wang Decheng, Zhao, Chao, Peng, Gang, Niu, Chen
- ID
- ZDB-PUB-210617-2
- Date
- 2017
- Source
- Journal of Microbes and Infections 12(3): 156-163 (Journal)
- Registered Authors
- Peng, Gang
- Keywords
- Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 Tumor necrosis factor α； Mutant zebrafish In situ hybridization Bacterial infection
- MeSH Terms
- none
- PubMed
- none
Citation
Xie, Lingling, Wang, Fenghua, Wang Decheng, Zhao, Chao, Peng, Gang, Niu, Chen (2017) Application of tumor necrosis factor α mutant zebrafish in Mycobacterium marinum infection. Journal of Microbes and Infections. 12(3):156-163.
Abstract
Abstract：Tumor necrosis factor α(TNF-α) is an important inflammatory cytokine implicated in the pathogenesis of rheumatoid arthritis, septic shock and other diseases. In order to investigate the functional role of host TNF-α in Mycobacterium marinum (M. marinum) infection, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9) technology was utilized to mutate the TNF-α gene in wild type (WT) zebrafish. A zebrafish containing a frame-shift mutation was successfully constructed, isolated, and named as TNF-α－/－. It was found that the expression of TNF-α mRNA was significantly reduced in TNF-α－/－ zebrafish by in situ hybridization (ISH). The proliferation of M. marinum in TNF-α－/－ zebrafish was significantly elevated compared to WT zebrafish. The construction of TNF-α－/－ zebrafish will allow further in-depth study on the function of TNF-α in mycobacterial infection and related immune regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping