PUBLICATION
Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes
- Authors
- Eom, D.S., Patterson, L.B., Bostic, R.R., Parichy, D.M.
- ID
- ZDB-PUB-210504-1
- Date
- 2021
- Source
- Developmental Biology 476: 314-327 (Journal)
- Registered Authors
- Eom, Dae Seok, Parichy, David M.
- Keywords
- Cell adhesion, Iridophore, JAM3, Melanophore, Pigment pattern, Zebrafish
- Datasets
- GEO:GSE131136
- MeSH Terms
-
- Animals
- Body Patterning/genetics*
- Carrier Proteins/metabolism
- Cell Adhesion Molecules/genetics
- Cell Adhesion Molecules/metabolism
- Gene Expression/genetics
- Gene Expression Regulation, Developmental/genetics
- Immunoglobulins/genetics
- Immunoglobulins/metabolism
- Junctional Adhesion Molecule C/genetics*
- Junctional Adhesion Molecule C/metabolism*
- Melanophores/metabolism
- Metamorphosis, Biological/genetics
- Morphogenesis
- Mutation/genetics
- Neural Crest/cytology
- Phenotype
- Pigmentation/genetics
- Signal Transduction/genetics
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 33933422 Full text @ Dev. Biol.
Citation
Eom, D.S., Patterson, L.B., Bostic, R.R., Parichy, D.M. (2021) Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes. Developmental Biology. 476:314-327.
Abstract
Adhesive interactions are essential for tissue patterning and morphogenesis yet difficult to study owing to functional redundancies across genes and gene families. A useful system in which to dissect roles for cell adhesion and adhesion-dependent signaling is the pattern formed by pigment cells in skin of adult zebrafish, in which stripes represent the arrangement of neural crest derived melanophores, cells homologous to melanocytes. In a forward genetic screen for adult pattern defects, we isolated the pissarro (psr) mutant, having a variegated phenotype of spots, as well as defects in adult fin and lens. We show that psr corresponds to junctional adhesion protein 3b (jam3b) encoding a zebrafish orthologue of the two immunoglobulin-like domain receptor JAM3 (JAM-C), known for roles in adhesion and signaling in other developing tissues, and for promoting metastatic behavior of human and murine melanoma cells. We found that zebrafish jam3b is expressed post-embryonically in a variety of cells including melanophores, and that jam3b mutants have defects in melanophore survival. Jam3b supported aggregation of cells in vitro and was required autonomously by melanophores for an adherent phenotype in vivo. Genetic analyses further indicated both overlapping and non-overlapping functions with the related receptor, Immunoglobulin superfamily 11 (Igsf11) and Kit receptor tyrosine kinase. These findings suggest a model for Jam3b function in zebrafish melanophores and hint at the complexity of adhesive interactions underlying pattern formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping