PUBLICATION

Calcineurin controls proximodistal blastema polarity in zebrafish fin regeneration

Authors
Cao, Z., Meng, Y., Gong, F., Xu, Z., Liu, F., Fang, M., Zou, L., Liao, X., Wang, X., Luo, L., Li, X., Lu, H.
ID
ZDB-PUB-210101-6
Date
2021
Source
Proceedings of the National Academy of Sciences of the United States of America   118(2): (Journal)
Registered Authors
Lu, Huiqiang, Luo, Lingfei
Keywords
calcineurin, fin regeneration, polarity, zebrafish
Datasets
GEO:GSE159560
MeSH Terms
  • Animal Fins/physiology*
  • Animals
  • Calcineurin/metabolism*
  • Cell Polarity/physiology
  • Extremities/physiology
  • Regeneration/physiology*
  • Signal Transduction
  • Wound Healing/physiology
  • Zebrafish/metabolism
  • Zebrafish Proteins
PubMed
33376206 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Planarian flatworms regenerate their heads and tails from anterior or posterior wounds and this regenerative blastema polarity is controlled by Wnt/β-catenin signaling. It is well known that a regeneration blastema of appendages of vertebrates such as fish and amphibians grows distally. However, it remains unclear whether a regeneration blastema in vertebrate appendages can grow proximally. Here, we show that a regeneration blastema in zebrafish fins can grow proximally along the proximodistal axis by calcineurin inhibition. We used fin excavation in adult zebrafish to observe unidirectional regeneration from the anterior cut edge (ACE) to the posterior cut edge (PCE) of the cavity and this unidirectional regeneration polarity occurs as the PCE fails to build blastemas. Furthermore, we found that calcineurin activities in the ACE were greater than in the PCE. Calcineurin inhibition induced PCE blastemas, and calcineurin hyperactivation suppressed fin regeneration. Collectively, these findings identify calcineurin as a molecular switch to specify the PCE blastema of the proximodistal axis and regeneration polarity in zebrafish fin.
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
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Errata and Notes