PUBLICATION
Nano-titanium nitride causes developmental toxicity in zebrafish through oxidative stress
- Authors
- Wang, J., Liu, K., Mo, C., Oleksandr, M.H., Zhang, Y., Chen, J.R., Hsiao, C.D., Zhu, Q., He, Q.
- ID
- ZDB-PUB-201212-11
- Date
- 2020
- Source
- Drug and chemical toxicology 45(4): 1660-1669 (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- Danio rerio, Nano-titanium nitride, Nrf2, development, oxidative stress
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Embryo, Nonmammalian/metabolism
- Oxidative Stress
- Reactive Oxygen Species/metabolism
- Water Pollutants, Chemical*/toxicity
- Zebrafish*
- PubMed
- 33297782 Full text @ Drug Chem. Toxicol.
Citation
Wang, J., Liu, K., Mo, C., Oleksandr, M.H., Zhang, Y., Chen, J.R., Hsiao, C.D., Zhu, Q., He, Q. (2020) Nano-titanium nitride causes developmental toxicity in zebrafish through oxidative stress. Drug and chemical toxicology. 45(4):1660-1669.
Abstract
Nano-titanium nitride (Nano-TiN) has strong resistance to wear and corrosion, good biocompatibility, and an attractive metallic luster. Nano-TiN is widely used in medical devices, such as orthopedic implants, syringe needles, coronary stents, and long-term dental implants, and also in imitation gold jewelry. Despite its widespread use, there are few reports describing safety evaluations of Nano-TiN. Here, we exposed healthy zebrafish embryos to different concentrations of Nano-TiN solution for five days, starting at about four hours post fertilization, and found that Nano-TiN caused dose- and time-dependent developmental toxicity. With increasing Nano-TiN concentration and length of exposure, mortality, and deformities gradually increased; body length shortened and hatching rate and motility were significantly reduced. We also found that exposure to Nano-TiN affected development of the heart, liver, nerves, and other organs, and led to elevated levels of reactive oxygen species and reduced antioxidant capacity. Exposure to Nano-TiN resulted in downregulation of expression of antioxidant genes, such as nrf2, gclc, gclm, ho-1, and nqo1. Our results showed that exposure to Nano-TiN caused developmental and organ toxicity in zebrafish embryos and that the toxic effects may be mediated through oxidative stress.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping