ZFIN ID: ZDB-PUB-201029-14
Growth Performance After Agouti-Signaling Protein 1 (Asip1) Overexpression in Transgenic Zebrafish
Godino-Gimeno, A., Sánchez, E., Guillot, R., Rocha, A., Angotzi, A.R., Leal, E., Rotllant, J., Cerdá-Reverter, J.M.
Date: 2020
Source: Zebrafish   17(6): 373-381 (Journal)
Registered Authors: Cerdá-Reverter, José Miguel, Rotllant, Josep
Keywords: growth, melanocortin, obesity, stress, transgenic, zebrafish
MeSH Terms:
  • Agouti Signaling Protein/genetics*
  • Agouti Signaling Protein/metabolism
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/growth & development
  • Crowding
  • Diet*
  • Gene Expression*
  • Obesity/genetics*
  • Stress, Physiological
  • Zebrafish/genetics
  • Zebrafish/growth & development*
PubMed: 33112719 Full text @ Zebrafish
The melanocortin system is a key structure in the regulation of energy balance. Overexpression of inverse agonists, agouti-signaling protein (ASIP), and agouti-related protein (AGRP) results in increased food intake, linear growth, and body weight. ASIP regulates dorsal-ventral pigment polarity through melanocortin 1 receptor (MC1R) and overexpression induces obesity in mice by binding to central MC4R. Asip1 overexpression in transgenic zebrafish (asip1-Tg) enhances growth, yet experiments show fish overexpressing Asip1 do not develop obesity even under severe feeding regimes. Asip1-Tg fish do not need to eat more to grow larger and faster; thus, increased food efficiency can be observed. In addition, asip1-Tg fish reared at high density are able to grow far more than wild-type (WT) fish reared at low density, although asip1-Tg fish seem to be more sensitive to crowding stress than WT fish, thus making the melanocortin system a target for sustainable aquaculture, especially as the U.S. Food and Drug Association has recently approved transgenic fish trading.