PUBLICATION

Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo

Authors
Dawes, M.L., Soeller, C., Scholpp, S.
ID
ZDB-PUB-201020-49
Date
2020
Source
Histochemistry and cell biology   154(5): 507-519 (Review)
Registered Authors
Scholpp, Steffen
Keywords
Fluorescent correlation spectroscopy (FCS), Microscopy, Protein–protein interactions, Zebrafish
MeSH Terms
  • Animals
  • Molecular Dynamics Simulation
  • Proteins/chemistry
  • Proteins/metabolism*
  • Spectrometry, Fluorescence
  • Zebrafish/embryology*
PubMed
33067656 Full text @ Histochem. Cell Biol.
Abstract
Cell behaviour and function is determined through the interactions of a multitude of molecules working in concert. To observe these molecular dynamics, biophysical studies have been developed that track single interactions. Fluorescence correlation spectroscopy (FCS) is an optical biophysical technique that non-invasively resolves single molecules through recording the signal intensity at the femtolitre scale. However, recording the behaviour of these biomolecules using in vitro-based assays often fails to recapitulate the full range of variables in vivo that directly confer dynamics. Therefore, there has been an increasing interest in observing the state of these biomolecules within living organisms such as the zebrafish Danio rerio. In this review, we explore the advancements of FCS within the zebrafish and compare and contrast these findings to those found in vitro.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes