PUBLICATION
In Vivo Pressurization of the Zebrafish Embryonic Heart as a Tool to Characterize Tissue Properties During Development
- Authors
- Gendernalik, A., Zebhi, B., Ahuja, N., Garrity, D., Bark, D.
- ID
- ZDB-PUB-201002-118
- Date
- 2020
- Source
- Annals of biomedical engineering 49(2): 834-845 (Journal)
- Registered Authors
- Garrity, Deborah
- Keywords
- Development, Embryo, Heart, Mechanical properties, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Heart/embryology*
- Heart/physiology*
- Models, Biological
- Stress, Mechanical
- Zebrafish/embryology
- PubMed
- 32959136 Full text @ Ann. Biomed. Eng.
Citation
Gendernalik, A., Zebhi, B., Ahuja, N., Garrity, D., Bark, D. (2020) In Vivo Pressurization of the Zebrafish Embryonic Heart as a Tool to Characterize Tissue Properties During Development. Annals of biomedical engineering. 49(2):834-845.
Abstract
Cardiac morphogenesis requires an intricate orchestration of mechanical stress to sculpt the heart as it transitions from a straight tube to a multichambered adult heart. Mechanical properties are fundamental to this process, involved in a complex interplay with function, morphology, and mechanotransduction. In the current work, we propose a pressurization technique applied to the zebrafish atrium to quantify mechanical properties of the myocardium under passive tension. By further measuring deformation, we obtain a pressure-stretch relationship that is used to identify constitutive models of the zebrafish embryonic cardiac tissue. Two-dimensional results are compared with a three-dimensional finite element analysis based on reconstructed embryonic heart geometry. Through these steps, we found that the myocardium of zebrafish results in a stiffness on the order of 10 kPa immediately after the looping stage of development. This work enables the ability to determine how these properties change under normal and pathological heart development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping