PUBLICATION
Development and migration of the zebrafish rhombencephalic octavolateral efferent neurons
- Authors
- Beiriger, A., Narayan, S., Singh, N., Prince, V.
- ID
- ZDB-PUB-200902-7
- Date
- 2020
- Source
- The Journal of comparative neurology 529(7): 1293-1307 (Journal)
- Registered Authors
- Beiriger, Anastasia, Prince, Victoria E.
- Keywords
- Hox genes, cell movement, facial nerve, hindbrain, lateral line system, neurons, zebrafish
- MeSH Terms
-
- Animals
- Cell Movement/physiology*
- Neurogenesis/physiology*
- Neurons, Efferent/physiology*
- Rhombencephalon/cytology*
- Rhombencephalon/physiology*
- Zebrafish
- PubMed
- 32869305 Full text @ J. Comp. Neurol.
Citation
Beiriger, A., Narayan, S., Singh, N., Prince, V. (2020) Development and migration of the zebrafish rhombencephalic octavolateral efferent neurons. The Journal of comparative neurology. 529(7):1293-1307.
Abstract
In vertebrate animals, motor and sensory efferent neurons carry information from the central nervous system (CNS) to peripheral targets. These two types of efferent systems sometimes bear a close resemblance, sharing common segmental organization, axon pathways, and chemical messengers. Here, we focus on the development of the octavolateral efferent neurons (OENs) and their interactions with the closely-related facial branchiomotor neurons (FBMNs) in zebrafish. Using live-imaging approaches, we investigate the birth, migration, and projection patterns of OENs. We find that OENs are born in two distinct groups: a group of rostral efferent neurons (RENs) that arises in the fourth segment, or rhombomere (r4), of the hindbrain and a group of caudal efferent neurons (CENs) that arises in r5. Both RENs and CENs then migrate posteriorly through the hindbrain between 18 and 48 hours post-fertilization, alongside the r4-derived FBMNs. Like the FBMNs, migration of the r4-derived RENs depends on function of the segmental identity gene hoxb1a; unlike the FBMNs, however, both OEN populations move independently of prickle1b. Further, we investigate whether the previously described 'pioneer' neuron that leads FBMN migration through the hindbrain is an r4-derived FBMN/REN or an r5-derived CEN. Our experiments verify that the pioneer is an r4-derived neuron and reaffirm its role in leading FBMN migration across the r4/5 border. In contrast, the r5-derived CENs migrate independently of the pioneer. Together, these results indicate that the mechanisms OENs use to navigate the hindbrain differ significantly from those employed by FBMNs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping