PUBLICATION

A bright and high-performance genetically encoded Ca2+ indicator based on mNeonGreen fluorescent protein

Authors
Zarowny, L., Aggarwal, A., Rutten, V.M.S., Kolb, I., Project, T.G., Patel, R., Huang, H.Y., Chang, Y.F., Phan, T., Kanyo, R., Ahrens, M.B., Allison, W.T., Podgorski, K., Campbell, R.E.
ID
ZDB-PUB-200624-3
Date
2020
Source
ACS sensors   5(7): 1959-1968 (Journal)
Registered Authors
Ahrens, Misha, Allison, Ted
Keywords
none
MeSH Terms
  • Animals
  • Calcium*
  • Cell Line
  • Cells, Cultured
  • Neurons*
  • Zebrafish*/genetics
PubMed
32571014 Full text @ ACS Sens
Abstract
Genetically encodable calcium ion (Ca2+) indicators (GECIs) based on green fluorescent proteins (GFP) are powerful tools for imaging of cell signaling and neural activity in model organisms. Following almost two decades of steady improvements in the Aequorea victoria GFP (avGFP)-based GCaMP series of GECIs, the performance of the most recent generation (i.e., GCaMP7) may have reached its practical limit due to the inherent properties of GFP. In an effort to sustain the steady progression towards ever-improved GECIs, we undertook the development of a new GECI based on the bright monomeric GFP, mNeonGreen (mNG). The resulting indicator, mNG-GECO1, is 60% brighter than GCaMP6s in vitro and provides comparable performance as demonstrated by imaging Ca2+ dynamics in cultured cells, primary neurons, and in vivo in larval zebrafish. These results suggest that mNG-GECO1 is a promising next-generation GECI that could inherit the mantle of GCaMP and allow the steady improvement of GECIs to continue for generations to come.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping