PUBLICATION

A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia

Authors
Kuncha, S.K., Mazeed, M., Singh, R., Kattula, B., Routh, S.B., Sankaranarayanan, R.
ID
ZDB-PUB-200522-31
Date
2018
Source
Nature communications   9: 511 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Alanine/chemistry*
  • Alanine/genetics
  • Alanine/metabolism
  • Amino Acid Sequence
  • Aminoacyltransferases/chemistry*
  • Aminoacyltransferases/genetics
  • Aminoacyltransferases/metabolism
  • Animals
  • Apicomplexa/genetics
  • Apicomplexa/metabolism
  • Bacteria/genetics
  • Bacteria/metabolism
  • Binding Sites
  • Biological Evolution
  • Cloning, Molecular
  • Crystallography, X-Ray
  • Gene Expression
  • Humans
  • Models, Molecular
  • Protein Binding
  • Protein Conformation, alpha-Helical
  • Protein Conformation, beta-Strand
  • Protein Interaction Domains and Motifs
  • RNA, Transfer, Amino Acyl/chemistry*
  • RNA, Transfer, Amino Acyl/genetics
  • RNA, Transfer, Amino Acyl/metabolism
  • Recombinant Proteins/chemistry
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Threonine/chemistry*
  • Threonine/genetics
  • Threonine/metabolism
  • Transfer RNA Aminoacylation/genetics*
PubMed
29410408 Full text @ Nat. Commun.
Abstract
D-aminoacyl-tRNA deacylase (DTD), a bacterial/eukaryotic trans-editing factor, removes D-amino acids mischarged on tRNAs and achiral glycine mischarged on tRNAAla. An invariant cross-subunit Gly-cisPro motif forms the mechanistic basis of L-amino acid rejection from the catalytic site. Here, we present the identification of a DTD variant, named ATD (Animalia-specific tRNA deacylase), that harbors a Gly-transPro motif. The cis-to-trans switch causes a "gain of function" through L-chiral selectivity in ATD resulting in the clearing of L-alanine mischarged on tRNAThr(G4•U69) by eukaryotic AlaRS. The proofreading activity of ATD is conserved across diverse classes of phylum Chordata. Animalia genomes enriched in tRNAThr(G4•U69) genes are in strict association with the presence of ATD, underlining the mandatory requirement of a dedicated factor to proofread tRNA misaminoacylation. The study highlights the emergence of ATD during genome expansion as a key event associated with the evolution of Animalia.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping