PUBLICATION
Tgfb3 collaborates with PP2A and Notch signaling pathways to inhibit retina regeneration
- Authors
- Lee, M., Wan, J., Goldman, D.
- ID
- ZDB-PUB-200513-9
- Date
- 2020
- Source
- eLIFE 9: (Journal)
- Registered Authors
- Goldman, Dan
- Keywords
- regenerative medicine, stem cells, zebrafish
- Datasets
- GEO:GSE145330
- MeSH Terms
-
- Animals
- Cell Proliferation
- Cellular Reprogramming
- Female
- Gene Expression Regulation
- Male
- Nerve Regeneration*
- Neuroglia/cytology
- Neuroglia/physiology*
- Protein Phosphatase 2/antagonists & inhibitors
- Protein Phosphatase 2/genetics
- Protein Phosphatase 2/metabolism*
- Receptor, Transforming Growth Factor-beta Type I/antagonists & inhibitors
- Receptor, Transforming Growth Factor-beta Type I/genetics
- Receptor, Transforming Growth Factor-beta Type I/metabolism
- Receptors, Notch/metabolism
- Retina/cytology
- Retina/physiology*
- Signal Transduction
- Smad3 Protein/genetics
- Smad3 Protein/metabolism
- Transforming Growth Factor beta3/genetics
- Transforming Growth Factor beta3/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32396062 Full text @ Elife
Citation
Lee, M., Wan, J., Goldman, D. (2020) Tgfb3 collaborates with PP2A and Notch signaling pathways to inhibit retina regeneration. eLIFE. 9:.
Abstract
Neuronal degeneration in the zebrafish retina stimulates Müller glia (MG) to proliferate and generate multipotent progenitors for retinal repair. Controlling this proliferation is critical to successful regeneration. Previous studies reported that retinal injury stimulates pSmad3 signaling in injury-responsive MG. Contrary to these findings, we report pSmad3 expression is restricted to quiescent MG and suppressed in injury-responsive MG. Our data indicates that Tgfb3 is the ligand responsible for regulating pSmad3 expression. Remarkably, although overexpression of either Tgfb1b or Tgfb3 can stimulate pSmad3 expression in the injured retina, only Tgfb3 inhibits injury-dependent MG proliferation; suggesting the involvement of a non-canonical Tgfb signaling pathway. Furthermore, inhibition of Alk5, PP2A or Notch signaling rescues MG proliferation in Tgfb3 overexpressing zebrafish. Finally, we report that this Tgfb3 signaling pathway is active in zebrafish MG, but not those in mice, which may contribute to the different regenerative capabilities of MG from fish and mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping