PUBLICATION
The Aqueous Extract from Ceratonia siliqua Leaves Protects Against 6-hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism
- Authors
- Abidar, S., Boiangiu, R.S., Dumitru, G., Todirascu-Ciornea, E., Amakran, A., Cioanca, O., Hritcu, L., Nhiri, M.
- ID
- ZDB-PUB-200422-84
- Date
- 2020
- Source
- Antioxidants (Basel, Switzerland) 9(4): (Journal)
- Registered Authors
- Keywords
- 6-hydroxydopamine, Ceratonia siliqua, aqueous extract, memory, oxidative stress
- MeSH Terms
- none
- PubMed
- 32276477 Full text @ Antioxidants (Basel)
Citation
Abidar, S., Boiangiu, R.S., Dumitru, G., Todirascu-Ciornea, E., Amakran, A., Cioanca, O., Hritcu, L., Nhiri, M. (2020) The Aqueous Extract from Ceratonia siliqua Leaves Protects Against 6-hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism. Antioxidants (Basel, Switzerland). 9(4):.
Abstract
Ceratonia siliqua L. is a Mediterranean medicinal plant traditionally cultivated for its ethnopharmacological benefits, such as antidiarrheal, antidiabetic, enhance acetylcholine, antioxidant, antiatherosclerotic, and for its possible anti-neurodegenerative potential. The aim of the present study was to evaluate the chemical composition, as well as the cognitive-enhancing, anxiolytic, and antioxidant activities of the aqueous extract from C. siliqua (CsAE) leaves against 6-hydroxydopamine (6-OHDA) zebrafish Parkinson's disease (PD) model. CsAE (0.1, 0.3, and 1 mg/L) was administered by immersion to zebrafish (Danio rerio) for eight consecutive days and one hour before each behavioral test of each day, while 6-OHDA (250 µM) treatment was supplied one day before the novel tank diving test (NTT). Qualitative and quantitative analyses were performed by the ultra-high-performance liquid chromatography (UHPLC) analysis. The memory performance was evaluated through the NTT and Y-maze tests. Additionally, the in vitro and in vivo antioxidant status and acetylcholinesterase (AChE) activity was also assessed. Our finds demonstrated that CsAE presented positive antioxidant and anti-AChE activities, which contributed to the improvement of cognitive function in the 6-OHDA zebrafish PD model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping