ZFIN ID: ZDB-PUB-200403-64
Copper induce zebrafish retinal developmental defects via triggering stresses and apoptosis
Zhao, G., Sun, H., Zhang, T., Liu, J.X.
Date: 2020
Source: Cell communication and signaling : CCS   18: 45 (Journal)
Registered Authors: Liu, Jing-xia, Zhang, Ting
Keywords: Apoptosis, ER, ROS, Retina, atp7a, cox17
MeSH Terms: none
PubMed: 32169084 Full text @ Cell Commun. Signal.
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ABSTRACT
The disorder of copper homeostasis is linked with disease and developmental defects, and excess copper_nanoparticles (CuNPs) and ion (Cu2+) will induce developmental malformation and disease in organisms. However, little knowledge is available regarding its potential regulation mechanisms, and little study links excess copper with retinal developmental malformation and disease.
Embryos were stressed with copper (CuNPs and Cu2+), and cell proliferation and apoptosis assays, reactive oxygen species (ROS) and endoplasmic reticulum (ER) signaling detections, and genetic mutants cox17-/- and atp7a-/- application, were used to evaluate copper induced retinal developmental malformation and the underlying genetic and biological regulating mechanisms.
Copper reduced retinal cells and down-regulated expression of retinal genes, damaged the structures of ER and mitochondria in retinal cells, up-regulated unfold protein responses (UPR) and ROS, and increased apoptosis in copper-stressed retinal cells. The copper induced retinal defects could be significantly neutralized by ROS scavengers reduced Glutathione (GSH) & N-acetylcysteine (NAC) and ER stress inhibitor 4- phenylbutyric acid (PBA). Blocking the transportation of copper to mitochondria, or to trans-Golgi network and to be exported into plasma, by deleting gene cox17 or atp7a, could alleviate retinal developmental defects in embryos under copper stresses.
This is probably the first report to reveal that copper nanoparticles and ions induce retinal developmental defects via upregulating UPR and ROS, leading to apoptosis in zebrafish embryonic retinal cells. Integrated function of copper transporter (Cox17 and Atp7a) is necessary for copper induced retinal defects.
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